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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1203-1210
DOI: https://doi.org/10.20546/ijcmas.2017.610.145


Comparative Evaluation of Changes in Protein Profile of Sugarcane Varieties under Different Soil Moisture Regimes
Pooja1*, A. S. Nandwal1, Mehar Chand2, Ashwani Kumar3, Babita Rani1, Anita Kumari1 and Neeraj Kulshrestha4
1CCS Haryana Agricultural University, Hisar – 125004, Haryana, India
2Regional Research Station, CCS HAU, Karnal – 132001, Haryana, India
3ICAR-Central Soil Salinity Research Institute, Karnal – 132001, Haryana, India
4ICAR-Sugarcane Breeding Institute, Regional Centre, Karnal – 132001, Haryana, India
*Corresponding author
Abstract:

Four commercial sugarcane varieties differing in their maturity were studied for the protein profiles under control (50% ASM), moisture stress of 40% ASM (mild stress) and 30 % ASM (severe stress). Both qualitative and quantitative differences were observed. Total soluble proteins content in leaves significantly increased at both 40% ASM levels (21.05 and 27.19%) and 30% (27.15 and 43.44%) as compared to 50% ASM level at 60 and 90 DAP, respectively. Severe stress of 30% ASM levels caused formation of new protein band of MW 18.56, 13.46, 31.6 and 36.6 kDa in leaves of variety Co 0238. In CoJ 64, one new polypeptide band of MW 38.5 kDa appeared and two polypeptide bands of MW 13.6 and 18.56 kDa disappeared. Variety CoS 767 showed the presence of four new band of 13.46, 15.6, 18.56 and 38.2 kDa. Likewise in variety CoH 128 two new polypeptide bands of MW 25.2 and 26.8 kDa appeared and 4 band of MW 13.46, 15.6, 18.17 and 54.6 kDa disappeared at 30% and 40% ASM levels. Appearance of new polypeptide(s) or their disappearance might be related to the genotypic stress tolerance or sensitivity.


Keywords: Available soil moisture, Water deficit, Polypeptides, Protein profile, Sugarcane.
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How to cite this article:

Pooja, A.S. Nandwal, Mehar Chand, Ashwani Kumar, Babita Rani, Anita Kumari and Neeraj Kulshrestha. 2017. Comparative Evaluation of Changes in Protein Profile of Sugarcane Varieties under Different Soil Moisture Regimes.Int.J.Curr.Microbiol.App.Sci. 6(10): 1203-1210. doi: https://doi.org/10.20546/ijcmas.2017.610.145