International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1112-1123

Optimization of Saccharification of Biological Pre-Treated Rice Straw by Response Surface Methodology
Pardeep Kaur1*, Gurvinder Singh Kocher1 and Monica Sachdeva Taggar2
1Department of Microbiology, Punjab Agricultural University, Ludhiana 141004, Punjab, India
2School of Renewable Energy Engineering, Punjab Agricultural University,Ludhiana 141004, Punjab, India
*Corresponding author

Pretreatment of rice straw facilitating the enzymatic saccharification process was performed by a ligninolytic fungal consortium, resulting in rice straw having 56.4 % cellulose, 12.2% hemicelluloses, and 2.2% lignin. Using response surface methodology, the combined effect of substrate concentration, temperature and pH on reducing sugar yield from enzymatic saccharification of rice straw optimized substrate concentration, 5.0% (w/v); temperature, 55°C and pH, 4.8 yielding a maximum sugar content of 492.0 milligram per gram dry substrate (mg/gds) with a saccharification efficiency of 81.1%. The experimental results were in good agreement with predicted values. Therefore, the model could be successfully used to identify the effective combinations of the three factors for predicting reducing sugar yield.

Keywords: Rice straw, Fungal consortium, Saccharification, Bioethanol, Response surface methodology.

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How to cite this article:

Pardeep Kaur, Gurvinder Singh Kocher and Monica Sachdeva Taggar. 2017. Optimization of Saccharification of Biological Pre-Treated Rice Straw by Response Surface Methodology.Int.J.Curr.Microbiol.App.Sci. 6(10): 1112-1123. doi:
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