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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 978-985
DOI: https://doi.org/10.20546/ijcmas.2017.610.118


Kinetics of Soil L-Glutaminase Enzyme
G .Pavani*, P. Chandrasekhar Rao, G. Padmaja and R. Subhash Reddy
Department of Soil Science and Agriculture Chemistry, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad – 500030, Telangana, India
*Corresponding author
Abstract:

An experiment was conducted in the Department of soil science and Agricultural chemistry, College of Agriculture, Rajendranagar, Hyderabad. The aim of this research work was to determine the kinetics of L-glutaminase in selected soil samples of Rangareddy district, Telengana state in the year 2014. The results show that the soil L-glutaminase activity increased sharply with increase in substrate concentration and almost reached a plateau at substrate concentrate of around 30 mM. The maximum reaction velocity of soil L-glutaminase (µg of NH4+ released g-1 soil 2h-1) varied from 12.65 to 58.82 and fallowed the sequence of VS 2> VS 1>AS 2>AS 1 under Line weaver – Burk plot. Under Hanes –Wolf plot the values varied from 22.72 to 45.45 and followed the sequence of VS1> VS 2> AS 2> AS 1. In Eadie – Hofstee the values varied from 16.25 to 19.84 and followed the sequence of AS 2> VS 1> AS 1> VS 2. The Michaelis - Menten constant varied from 8.23 to 82.41 and follows the sequence VS 2> VS 1> AS 2> AS 1. Michaelis - Menten constant expressed in mM and these values compared well with those obtained from Hanes-Wolf and Eadie - Hofstee plots. Under the present study, the Km and Vmax values for the Vertisols were high compared to Alfisols. Though the clay content of Vertisols was observed to be low, the presence of montmorillonite type of clay which absorbed the enzyme in its interlayer spaces. Hence it has expressed maximum enzyme activity in these soils.


Keywords: Kinetics, Soil, Enzyme, Telangana, Weaver.
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How to cite this article:

Pavani, G., P. Chandrasekhar Rao, G. Padmaja and Subhash Reddy, R. 2017. Kinetics of Soil L-Glutaminase EnzymeInt.J.Curr.Microbiol.App.Sci. 6(10): 978-985. doi: https://doi.org/10.20546/ijcmas.2017.610.118