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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 342-349
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.035


Generation and Characterization of Monoclonal Antibodies Specific to Burkholderia mallei
Sudhir K. Dohre, Urmil Tuteja and Subodh Kumar*
Defence Research & Development Establishment, Gwalior - 474002, Madhya Pradesh, India
*Corresponding author
Abstract:

Glanders is a contagious disease of equines and is endemic in many countries. There are problems with existing glanders diagnostic tests and monoclonal antibody (MoAbs) based tests can be useful. The aim of this study was to generate MoAbs specific to Burkholderia mallei. BALB/c mice were used for the generation of MoAb and were injected with whole cell sonicated antigen (WCS) of B. mallei. After 3 injections, two final boosts were given and spleen cells of mouse showing the highest antibody titer to WCS antigen by ELISA collected and fused with myeloma cells in presence of PEG 2000. Twenty nine hybridomas producing antibodies were generated of which five stable clones were expanded, among them three clones were finally checked for the specificity of their antibodies against various Burkholderia species and other closely related bacteria. Two of the MoAbs DM01BUMA01 and DM01BUMA02 did not show any cross reaction with any of the tested bacteria, DM01BUMA03 cross reacted with B. cenocepacia and B. gladoili. The MoAbs can be utilized for detection of B. mallei.


Keywords: Burkholderia mallei,Glanders,monoclonal antibodies (MoAbs).
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How to cite this article:

Sudhir K. Dohre, Urmil Tuteja and Subodh Kumar. 2016. Generation and Characterization of Monoclonal Antibodies Specific to Burkholderia mallei.Int.J.Curr.Microbiol.App.Sci. 5(5): 342-349. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.035