National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was planned to evaluate the anthelmintic efficacy of crude powder (CP), crude aqueous (CAE) and crude methanolic (CME) extracts of Nicotiana tabacum leaves against common gastrointestinal nematodes of goats using three in-vitro tests viz. adult motility test (AMT), egg hatch assay (EHA) and larval paralysis test (LPT) and one in-vivo test using faecal egg count reduction test (FECRT). AMT was conducted against freshly collected adult Haemonchus contortus. However, EHA and LPT tests were conducted against eggs and larvae of gastrointestinal nematodes, respectively of goats or AMT at different concentrations viz.0.156%, 0.312%, 0.625%, 1.25%, 2.5%, 5% and 10%. In-vivo trial was also conducted in goats naturally infected with mixed infection of GI nematodes using FECRT. In AMT, an average highest corrected motility of 100% was recorded from 0.625–10% concentration followed by 66.66% at 0.156 and 0.312% concentrations. Overall CP and CAE showed maximum (100%) adulticidal activity against H. contortus and minimum (71.43%) with CME. In EHA, CME of N. tabacum showed better ED50=0.522 and ED99= 21.32mg/ml in comparison to CAE (ED50=0.40 and ED99= 36.22mg/ml). The highest (100%) inhibition of egg hatching was observed with CAE @ 50 and 100mg/ml and CME @ 25, 50 and 100 mg/ml concentration. In case of LPT, crude methanolic extract has better (ED50= 0.69 and ED99= 9.58 mg/ml) values than crude aqueous extract (ED50= 0.759 and ED99= 14.9349 mg/ml). The highest (100%) paralysis of third stage larvae was observed with CAE @ 25, 50 and 100mg/ml and CME @12.5, 25, 50 and 100mg/ml concentration. In in-vivo trial, the highest (100%) reduction in faecal egg count was recorded against CAE @ both 100 and 200mg/kg b. wt. 14 days post-treatment. However, CME and CP both showed maximum effect of 33.33% and 15.79%, respectively @200mg/kg b. wt. on 14 days post-treatment.
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