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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 322-341
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.034


Effect of Organophosphate Insecticide, Dimethoate on Physiology of Common carp, Catla catla (Hamilton) and Labeo rohita
Mohammad Illiyas Hussain1, Baidyanath Kumar2* and Mumtaz Ahmad3
1Research Scholar, Department of Zoology, Oriental College, Patna, India
2Visiting Professor, Department of Biotechnology, College of Commerce, Patna, India
3Head, Department of Zoology, Oriental College, Patna, India
*Corresponding author
Abstract:

In the present investigation  the effects of Dimethoate insecticide on survival chance (acute toxicity), behavioral response and blood biochemical and haematological parameters and the expression of isoenzymes in Catla catla (Hamilton) and Labeo rohita (Hamilton) after exposure to sublethal concentrations of Dimethoate was studied. It is evident that no mortality of Catla catla and Labeo rohita was recorded at 20.5mg/l Dimethoate up to 24 hrs. of exposure. At concentrations of 23.0, 23.5 and 24.0mg/l of Dimethoate 100% mortality of Catla catla was recorded at three different concentration of Dimethoate viz., 23.0mg/l, 23.5mg/l and 24.0mg/l in 96 hrs. of exposure. The LC50 values of Dimethoate at various exposure times were 21.0mg/l for about 84 hrs; 21.5mg/l for 72 hrs; 22.0mg/l for about 60hrs; 22.5mg/l for 48 hrs; 23.0mg/l for 48 hrs; and 23.5mg/l for 24 hrs. The percentage mortality of Catla catla increased with increased concentration of Dimethoate and with decreased exposure time. Similarly at concentrations of 23.0, 23.5 and 24.5mg/l of Dimethoate 100% of mortality of Labeo rohita was recorded. Catla catla and Labeo rohita showed behavioral alterations against Dimethoate intoxication. Uncoordinated movements, erratic swimming, convulsions, excess mucus secretion, decreased opercular movements, loss of balance, drowning and change in body pigmentation, muscle fasciculation. Moribund lethargy, refusal of feeding, respiratory distress became more apparent with increase in duration of exposure at all test concentration of Dimethoate. The glycogen content, total proteins and total lipids decreased in the blood of Catla catla and Labeo rohita under the toxicity of Dimethoate whereas the levels of total free sugars and total free amino acids increased. Dimethoate caused reduction in total proteins in the blood of both Catla catla and Labeo rohita. The acute exposure to Dimethoate resulted in a significant decrease in count of developmental forms of myeloid sequence and the segmented neutrophilic granulocytes in the experimental group. Catla catla and Labeo rohita exposed to acute effects of the Dimethoate insecticide showed significantly (P < 0.01) decreased concentration of alkaline phosphatase and significantly (P < 0.01) increased concentration of ammonia, aspartate aminotransferase, lactate dehydrogenase,creatine kinase and lactate in blood plasma. The main biochemical blood profile response of Catla catla and Labeo rohita to the acute effect of 23.5 mg/l of Dimethoate was a significantly (P < 0.01) decreased concentration of alkaline phosphatase and significantly (P < 0.01) increased concentration of NH3, LDH, AST, CK and LACT in blood plasma.


Keywords: LC50,Dimethoate,Catla catla,Labeo rohita Toxicity, Serum composition, Isoenzymes.
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How to cite this article:

Mohammad Illiyas Hussain, Baidyanath Kumar and Mumtaz Ahmad. 2016. Effect of Organophosphate Insecticide, Dimethoate on Physiology of Common carp, Catla catla (Hamilton) and Labeo rohitaInt.J.Curr.Microbiol.App.Sci. 5(5): 322-341. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.034