Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 586-594
DOI: https://doi.org/10.20546/ijcmas.2017.610.072


A Fast Protocol for in vitro Cloning of Banana (Musa acuminata) cv. Amritpani
Kaberi Maharana1, Sashikala Beura2* and Partha Sarathi Munsi1
1Department of Horticulture and Post-Harvest Technology, Institute of Agriculture,Visva-Bharti, Sriniketan, West Bengal-731236, India
2Department of Floriculture and Landscaping and Biotechnology-cum-Tissue Culture Centre, College of Agriculture, OUAT, Bhubaneswar, Odisha-751003, India
*Corresponding author
Abstract:

An investigation has been conducted on Fast Protocol for in vitro cloning of Banana (Musa acuminata) cv. Amritpani at the Biotechnology-cum-Tissue Culture Center, OUAT, Bhubaneswar, during the year 2014-16. This has helped to determine the best media compositions for callus formation, shoot initiation, shoot proliferation and rooting of banana cv. Amritpani, so as to get optimum results with a minimized cost of productions. MS medium supplemented by 7.0 mg/l + 0.5 mg/l IAA, significantly enhance the callus production, MS medium fortified by 5.0 mg/l + 0.5 mg/l IAA was found to be most potent combination and produced a significantly higher number of multiple shoots per clump (29.77), after 30 days of culturing. The same combination recorded significantly higher number of shoots/explants. i.e., 30.03 and 12.33 in 1st and 2nd sub-culturing respectively. MS medium fortified with 1.0 mg/l IBA was found to be ideal and produced maximum number of roots/plants (5.08) and average root length (5.30 cm). This protocol might be used for the massive in vitro production of plantlets of banana cv. Amritpani.


Keywords: In vitro, inoculation, Musa acuminate, Shoot proliferation.
Download this article as Download

How to cite this article:

Kaberi Maharana, Sashikala Beura and Partha Sarathi Munsi. 2017. A Fast Protocol for in vitro Cloning of Banana (Musa acuminata) cv. AmritpaniInt.J.Curr.Microbiol.App.Sci. 6(10): 586-594. doi: https://doi.org/10.20546/ijcmas.2017.610.072