International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 586-594

A Fast Protocol for in vitro Cloning of Banana (Musa acuminata) cv. Amritpani
Kaberi Maharana1, Sashikala Beura2* and Partha Sarathi Munsi1
1Department of Horticulture and Post-Harvest Technology, Institute of Agriculture,Visva-Bharti, Sriniketan, West Bengal-731236, India
2Department of Floriculture and Landscaping and Biotechnology-cum-Tissue Culture Centre, College of Agriculture, OUAT, Bhubaneswar, Odisha-751003, India
*Corresponding author

An investigation has been conducted on Fast Protocol for in vitro cloning of Banana (Musa acuminata) cv. Amritpani at the Biotechnology-cum-Tissue Culture Center, OUAT, Bhubaneswar, during the year 2014-16. This has helped to determine the best media compositions for callus formation, shoot initiation, shoot proliferation and rooting of banana cv. Amritpani, so as to get optimum results with a minimized cost of productions. MS medium supplemented by 7.0 mg/l + 0.5 mg/l IAA, significantly enhance the callus production, MS medium fortified by 5.0 mg/l + 0.5 mg/l IAA was found to be most potent combination and produced a significantly higher number of multiple shoots per clump (29.77), after 30 days of culturing. The same combination recorded significantly higher number of shoots/explants. i.e., 30.03 and 12.33 in 1st and 2nd sub-culturing respectively. MS medium fortified with 1.0 mg/l IBA was found to be ideal and produced maximum number of roots/plants (5.08) and average root length (5.30 cm). This protocol might be used for the massive in vitro production of plantlets of banana cv. Amritpani.

Keywords: In vitro, inoculation, Musa acuminate, Shoot proliferation.

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Kaberi Maharana, Sashikala Beura and Partha Sarathi Munsi. 2017. A Fast Protocol for in vitro Cloning of Banana (Musa acuminata) cv. AmritpaniInt.J.Curr.Microbiol.App.Sci. 6(10): 586-594. doi:
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