International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 522-526

Detection of Biofilm Formation in Multi-Drug Resistant Staphylococcus aureus from Rural Tertiary Care Hospital in North India
Chinithung Ngullie*, Ashma Khatun, Varsha A. Singh, Nitasha and Rajdeep Paul
Department of Microbiology, MMIMSR, Mullana, Ambala, Haryana, 133207, India
*Corresponding author

One of the hallmark characteristic of Staphylococcus aureus infection is its inherent ability to form biofilm on abiotic and abiotic surfaces. Biofilm producing Organisms are notoriously hard to treat owing to their high resistance against commonly used antibiotics. This study aimed at Detection of biofilm formation by Multi-drug resistant Staphylococcus aureus strain resistant to methicillin and Ciprofloxacin from clinical samples. The objectives of the study were: To detect biofilm formation by multi-drug resistant Staphylococcus aureus isolated from clinical specimens by three different methods: Tissue Culture Plate (TCP), Tube Adherence Method (TAM) and Modified Congo Red Agar (MCRA) method. The main objective is to determine the sensitivity, specificity, positive predictive value and negative predictive value of MCRA and TAM against TCP method. A total of 50 Staphylococcus aureus strains resistance to methicillin and Ciprofloxacin from all clinical isolates from April 2015 to March 2016 was subjected to biofilm detection methods. Out of 50 S. aureus resistant to methicillin and ciprofloxacin, 26(52%) were found to be biofilm producers. Rate of Detection of biofilm was highest by Modified Congo red agar (42%) and Tube Adherence method (38%) followed by Tissue Culture plate method (32%). Rate of detection by three different methods in combination was 42.3% and decreased as per number of tests. In the present study the rate of biofilm producers detected by all three methods was 26(52%). Among which, Modified Congo Red Agar Method was best 21(42%) followed by Tube adherence method 19(38%) and Tissue Culture Plate Method 16 (32%). Rate of detection by combination of all three methods was 52%. Hence, this study endorses for frequent detection of Biofilm production by S. aureus especially by multi-drug resistant organisms.

Keywords: Biofilm, Multi-drug resistant Staphylococcus aureus.

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How to cite this article:

Chinithung Ngullie, Ashma Khatun, Varsha A. Singh, Nitasha and Rajdeep Paul. 2017. Detection of Biofilm Formation in Multi-Drug Resistant Staphylococcus aureus from Rural Tertiary Care Hospital in North IndiaInt.J.Curr.Microbiol.App.Sci. 6(10): 522-526. doi:
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