International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 302-313

Assessment of Metal Chelating, Bioautography and Spot Screening by TLC of Green Tea (Camellia sinensis)
Arti Ghabru1* and R.G. Sud2
1Department of Basic Sciences, College of Forestry, UHF, Nauni, Solan, HP, India
2Department of Chemistry and Biochemistry, COBS, CSKHPV, Palampur, HP, India
*Corresponding author

Tea is receiving increased interest from scientists due to its biological properties and the presence of bioactive substances like polyphenols which give nutraceutical values to well brew cup of tea. The study was carried out as a prerequisite to evaluate the therapeutic potential of green tea. The antioxidant potential of Kangra tea in terms of metal ions chelation was determined. Ferrous ions chelation potential of tea powders (in terms of IC50 (Fe2+) values) was in between the IC50 (Fe2+) values of EDTA and citric acid. The IC50 (Fe2+) exhibited a correlation with TC and flavanols. A comparative TLC (Thin layer chromatography) bioautograph study between aqueous solutions of standard catechins (0.5 mg mL-1) and tea powder (1 mg mL-1) indicated that EGCG was one of the active components present in tea solution which might be responsible for antibacterial activity of Kangra tea. Growth inhibition of bacterial pathogens (B. cereus and S. aureus) by aqueous solution of tea powder was found to be influenced by the addition of ferrous ions which suggest that the antibacterial activity of tea catechins could be due to their ability to chelate ferrous ions, indicating that this activity is bacteriostatic rather than bactericidal in nature.

Keywords: Green tea, Metal Chelating ions, Antibacterial assay, Antioxidant activity, Phytochemical screening,Camellia sinensis.

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How to cite this article:

Arti Ghabru and Sud, R.G. 2017. Assessment of Metal Chelating, Bioautography and Spot Screening by TLC of Green Tea (Camellia sinensis).Int.J.Curr.Microbiol.App.Sci. 6(10): 302-313. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.