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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 209-215
DOI: https://doi.org/10.20546/ijcmas.2017.610.026


Detection of Beta-Lactam Resistance in Piscean Escherichia coli using Combination Disc Method and Multiplex PCR
M. Soma Sekhar1*, N. Mohammad Sharif2, T. Srinivasa Rao1 and Ch. Bindu Kiranmayi1
1Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, India
2Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India
*Corresponding author
Abstract:

Intestinal contents from freshwater fish (Catla catla) (n=150) were bacteriological cultured for Escherichia coli and confirmed by PCR targeting E16S gene. A total of 104 E. coli isolates were recovered and analyzed for the presence of extended-spectrum beta-lactamases (ESBLs), phenotypically by disc diffusion method and genotypically by multiplex PCR targeting blaTEM, blaSHV, blaOXA, blaCTX-M and blaAmpC beta-lactamase (bla) genes. Phenotypic beta-lactam resistance was detected in a total of 16 isolates, of which nine isolates carried ESBL phenotype by combination disc method. Multiplex PCR assay revealed presence of blaAmpC, blaTEM, blaCTX-M, blaSHV and blaOXA genes in 13, 13, 9, 6 and 4 isolates, respectively. Serotyping of beta-lactam resistant E. coli isolates (n=16) revealed O120 (2 isolates), O141 (2), rough (2), O63 (1), O126 (1) and untypable (8) serotypes. This was the first report on the phenotypic detection and molecular confirmation of ESBL producing E. coli from freshwater fish in Andhra Pradesh.


Keywords: Beta-lactam resistance, ESBL, E. coli, E16S, Freshwater fish.
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How to cite this article:

Soma Sekhar, M., N. Mohammad Sharif, T. Srinivasa Rao and Bindu Kiranmayi, Ch. 2017. Detection of Beta-Lactam Resistance in Piscean Escherichia coli using Combination Disc Method and Multiplex PCR.Int.J.Curr.Microbiol.App.Sci. 6(10): 209-215. doi: https://doi.org/10.20546/ijcmas.2017.610.026