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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 119-122
DOI: https://doi.org/10.20546/ijcmas.2017.610.014


Clinical and Molecular Diagnosis of Johne’s Disease in a Jamunapari Goat – A Case Report
P. Kanimozhi, M. Geetha*, K.M. Palanivel and M. Arthanari Easwaran
Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India
*Corresponding author
Abstract:

A 2 year old male Jamunapari goat weighing 30 kilograms was presented with complaints of chronic diarrhea, progressive weight loss and poor response to therapy. Faecal sample was collected aseptically and subjected for the presence of Mycobacterium avium subspecies paratuberculosis (MAP) by Ziehl-Neelsen staining and polymerase chain reaction (PCR). Ziehl – Neelsen staining (ZN) revealed the presence of clumped acid fast bacilli in the faecal smears. For further differencing between Acid fast bacilli from nonacid fast MAP confirmation, DNA was extracted from faecal sample and subjected to PCR by using specific primers targeting IS 900 gene sequence of MAP and confirmed JD by detection of 413 bp PCR product.


Keywords: Johne’s diseaes, Goat, IS 900 PCR.
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How to cite this article:

Kanimozhi, P., M. Geetha, K.M. Palanivel and Arthanari Easwaran, M. 2017. Clinical and Molecular Diagnosis of Johne’s Disease in a Jamunapari Goat – A Case Report.Int.J.Curr.Microbiol.App.Sci. 6(10): 119-122. doi: https://doi.org/10.20546/ijcmas.2017.610.014