National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A 2 year old male Jamunapari goat weighing 30 kilograms was presented with complaints of chronic diarrhea, progressive weight loss and poor response to therapy. Faecal sample was collected aseptically and subjected for the presence of Mycobacterium avium subspecies paratuberculosis (MAP) by Ziehl-Neelsen staining and polymerase chain reaction (PCR). Ziehl – Neelsen staining (ZN) revealed the presence of clumped acid fast bacilli in the faecal smears. For further differencing between Acid fast bacilli from nonacid fast MAP confirmation, DNA was extracted from faecal sample and subjected to PCR by using specific primers targeting IS 900 gene sequence of MAP and confirmed JD by detection of 413 bp PCR product.
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