National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Thirty two Rhizoctonia solani isolates were studied for molecular variability by RAPD technique at the Institute of Agri-biotechnology, University of Agricultural Sciences, Dharwad, India during 2007. The results indicated that, there were differences in RAPD profiles of 32 isolates of R. solani with two random primers OPB-15 and OPD-13. The primer OPB-15 and OPD-13 produced 248 PCR products of molecular weight ranging from 300 bp to 600 bp in different isolates with diverse amplicon. To test the resolving ability of these primers, cumulative RAPD profiles generated by the random primers were analyzed by UPGMA. All the isolates shared minimum of 60 per cent and above similarity among themselves. The cumulative analysis of 32 isolates showed four groups at 75 per cent genetic similarity level.
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