International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 112-118

Genetic Variability in the Isolates of Rhizoctonia solani Kühn, the Causal Agent of Sheath Blight of Rice by Using RAPD Markers
P. Nagaraju1* and M.K. Naik2
1Plant Pathology, AICRP on Groundnut, University of Agricultural Sciences,Dharwad-580 005, Karnataka, India
2Department of Plant Pathology, University of Agricultural Sciences, Raichur-584 104, Karnataka, India
*Corresponding author

Thirty two Rhizoctonia solani isolates were studied for molecular variability by RAPD technique at the Institute of Agri-biotechnology, University of Agricultural Sciences, Dharwad, India during 2007. The results indicated that, there were differences in RAPD profiles of 32 isolates of R. solani with two random primers OPB-15 and OPD-13. The primer OPB-15 and OPD-13 produced 248 PCR products of molecular weight ranging from 300 bp to 600 bp in different isolates with diverse amplicon. To test the resolving ability of these primers, cumulative RAPD profiles generated by the random primers were analyzed by UPGMA. All the isolates shared minimum of 60 per cent and above similarity among themselves. The cumulative analysis of 32 isolates showed four groups at 75 per cent genetic similarity level.

Keywords: Genetic variability, Rhizoctonia solani isolates, RAPD markers, Sheath blight, Rice.

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How to cite this article:

Nagaraju, P. and Naik, M.K. 2017. Genetic Variability in the Isolates of Rhizoctonia solani Kühn, the Causal Agent of Sheath Blight of Rice by Using RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 6(10): 112-118. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.