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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 87-93
DOI: https://doi.org/10.20546/ijcmas.2017.610.010


Evaluation of Sperm Viability and Acrosomal Integrity by Flow Cytometry Analysis in Jersey Crossbred Bulls
V. Rajaram1, A. Palanisammi2*, V. Maroudam3, S.N. Sivaselvam4 and K. Kumanan5
1Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-7, Tamil Nadu, India
2Education Cell, Madras Veterinary College, TANUVAS, Chennai-7, Tamil Nadu, India
3Translational Research Platform for Veterinary Biologicals, Chennai – 51, Tamil Nadu, India
4Madras Veterinary College, TANUVAS, Chennai-7, Tamil Nadu, India
5Centre for Animal Health Studies, TANUVAS, Chennai-51, Tamil Nadu, India
*Corresponding author
Abstract:

Flow cytometry based evaluation of sperm parameters is advantageous over the traditional microscope based evaluation. Many sperm parameters can be measured within short time period and with high accuracy. For this study, semen from six Jersey crossbred bull were collected. Semen parameters like sperm for viability and acrosomal integrity were analyzed by flow cytometry. Sperm for viability measured by the propidium iodide staining indicated that Bull No: 3 have the highest number of viable spermatozoa with 82.89 per cent followed by 82.18, 80.26, 79.43, 75.20 and 70.73 for Bull No: 1, 5, 2, 6 and 4 respectively. Acrosomal integrity of the sperm sample were determined by fluorescein isothiocynate (FITC) labelled with Peanut agglutinin (PNA) staining. The percentage of viable sperm, non-viable sperm, viable intact sperm, viable damage sperm and viable damage and non-viable sperm was ranged from 75.12 to 82.28, 17.72 to 30.66, 58.55 to 78.48, 3.80 to 10.80 and 21.52 to 41.45 respectively. Chi’s square analysis of the semen samples indicated that there was a significant difference among bulls with respect to both the sperm viability and acrosomal integrity (P<0.01).


Keywords: Jersey crossbred bulls, Fertility, Flow cytometry, Sperm for viability, Acrosomal integrity.
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How to cite this article:

Rajaram, V., A. Palanisammi, V. Maroudam, S.N. Sivaselvam and Kumanan, K. 2017. Evaluation of Sperm Viability and Acrosomal Integrity by Flow Cytometry Analysis in Jersey Crossbred Bulls.Int.J.Curr.Microbiol.App.Sci. 6(10): 87-93. doi: https://doi.org/10.20546/ijcmas.2017.610.010