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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 194-204
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.021


Comparison of E-test and Colorimetric Micro Broth Dilution Method for Antifungal Susceptibility Testing of Candida Isolates
Prasanna1*, Partha Roy2, Raghu Sriram3, Naveen Grover3, Priyanka Pandit4 and Mayuri A Kulkarni5
1Department of Microbiology, Shri SathyaSai Medical College & Research Institute, Ammapettai, Kancheepuram- 608103, India
2Department of Microbiology, Delhi Base Hospital, Delhi, India
3Department of Microbiology, AFMC, Pune-411040, India
4Department of Pathology, Command Hospital, Kolkata, India
5Department of Pathology, Dr Hedgewar Hospital, Aurangabad, India
*Corresponding author
Abstract:

E strips used in Epsilometer test (E-test) for antifungal susceptibility testing is a simple, convenient and promising method with broad applications in microbiology. This E-test obtains a reproducible and quantitative MIC reading, with the E strips used that contain a predefined continuous gradient of a drug. The main aim of this study includes comparing the antifungal susceptibility testing of Candida species by E-test and Colorimetric Sensititre YeastOne micro broth dilution (CMBD) methods.   This was a cross sectional study, carried out from December 2012 to Nov 2014 from hospitalized patients at a tertiary care centre, in which 50 non-repetitive clinical isolates of Candida were analysed. The antifungal susceptibility testing (AFST) of these isolates was carried out by E-test and Colorimetric micro broth dilution (Sensititre Yeast One- TREK diagnostics), MIC values were interpreted as per CLSI M27-S4 (2012). Out of 50 isolates, 36 (72%) were non-albicans Candida and 14 (28%) were C.albicans. AFST by E-test showed that 24 isolates (48%) were resistant to fluconazole and voriconazole but all were sensitive to amphotericin B. Out of 24 resistant isolates by E-test 12 isolates were subjected to CMBD, showed all were resistant to fluconazole and voriconazole and 05 were sensitive to other azoles (posaconazole, Itraconazole, ketoconazole) and all isolates were sensitive to amphotericin B, caspofungin and 5-flucytosine. We conclude that E-test is the simple, convenient and promising method for antifungal susceptibility testing with excellent correlation with CMBD.


Keywords: Candida, E-test, Colorimetric  micro broth dilution,Sensititre Yeast One.
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How to cite this article:

Prasanna, S., Partha Roy, Raghu Sriram, Naveen Grover, Priyanka Pandit and Mayuri A Kulkarni. 2016. Comparison of E-test and Colorimetric Micro Broth Dilution Method for Antifungal Susceptibility Testing of Candida Isolates.Int.J.Curr.Microbiol.App.Sci. 5(5): 194-204. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.021