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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3457-3463
DOI: https://doi.org/10.20546/ijcmas.2017.609.424


Screening and Characterization of Rhizospheric Chitinolytic Bacteria for Evaluation of Their Potential as Biocontrol Agent against Phytopathogenic Fungi
Abhilipsa Parhi and Sukantibala Mohapatra*
Department of Microbiology, C.B.S.H., Orissa University of Agriculture and Technology, Bhubaneswar, Odisha-751003, India
*Corresponding author
Abstract:

The present study evaluated the antagonistic effect of the rhizospheric chitinolytic bacterial isolates of rice and green gram soil samples against three phytopathogenic fungi, i.e. Pythium aphanidermatum (ITCC No.-5488), Fusarium oxysporum (ITCC No.-4998) and Rhizoctonia solani (ITCC No.-2060). A total of 25 bacterial isolates were obtained based upon their distinct colony characteristics on colloidal chitin agar medium. Only 6 isolates (G1, G2, R3, R4, R9 and R10) showed visible antagonistic activity against the test fungi by dual culture method on potato dextrose agar (PDA) during primary screening. During further screening by well diffusion method, all the 6 showed broad-spectrum antibacterial activity by inhibiting most of the test fungal phytopathogens. The six effective isolates were also able to reduce the fungal biomass of all the three test pathogens by mycelial reduction test. Though R10 inhibited all the three fungal pathogens but maximum reduction was observed against Rhizoctonia solani. Physiological & biochemical characterization of the effective isolates indicated that the antagonistic chitinolytic bacterial belong to Bacillus sp.


Keywords: Chitinolytic bacteria, Antagonistic activity, Phytopathogenic fungi, Rhizoctonia solani, Bacillus sp.
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How to cite this article:

Abhilipsa Parhi and Sukantibala Mohapatra. 2017. Screening and Characterization of Rhizospheric Chitinolytic Bacteria for Evaluation of Their Potential as Biocontrol Agent against Phytopathogenic Fungi.Int.J.Curr.Microbiol.App.Sci. 6(9): 3457-3463. doi: https://doi.org/10.20546/ijcmas.2017.609.424