National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study was designed to investigate the role of the outer membrane proteins as a mechanism of resistance in Klebsiella isolates collected from Tanta university Hospital in Tanta, Egypt and to correlate between the Outer membrane proteins (OMPs) profiles and the resistance patterns of the tested isolates. The outer membrane of gram-negative bacteria plays a significant role in a variety of functions and is composed of a bilayer containing phospholipids, lipopolysaccharide and outer membrane proteins (OMPs). Two major non-specific porins, namely Omp K35 and Omp K36 have been described in Klebsiella pneumoniae. A total of 13 multidrug-resistant (MDR) Klebsiella isolates were obtained. Antibiotic susceptibility test was performed by disk diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution method for the selected antibiotics. Analysis of the outer membrane profile was performed on 12% separating acrylamide gel and vertical polyacrylamide gel electrophoresis (PAGE) apparatus. It was found that The relation between the resistance patterns and OMPs profiles of the tested isolates was difficult to be determined as strains having the same resistance pattern may exhibit different OMPs profiles and vice versa
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