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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 2914-2924
DOI: https://doi.org/10.20546/ijcmas.2017.609.358


Prebiotic Treatment Influence the Adhesion Properties of Three Lactobacillus strains
Pavlina Sredkova1, Ilia Iliev2, Balik Dzhambazov1 and Tsvetelina Batsalova1*
1Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, 24 TzarAsen Str., 4000 Plovdiv, Bulgaria
2Department of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv “PaisiiHilendarski”, 24 TzarAsen Str., 4000 Plovdiv, Bulgaria
*Corresponding author
Abstract:

Adhesion to intestinal epithelial cells is commonly analyzed during investigations on potential probiotic activity of certain bacteria. The aim of the present study was to investigate whether different prebiotics affect the adhesion properties of three strains lactobacilli (Lactobacillus rhamnosus 1010, Lactobacillus acidophilus11, and Lactobacillus paracasei 8458). Using in vitro model system based on cell lines derived from colon epithelial cells, we have determined the adherence ability of the three bacterial strains following treatment with 6 different prebiotics (inulin, pectin, chitosan, galacto-oligosaccharides, xylooligosaccharides and beta-glucan). Our results demonstrated significant reduction of lactobacilli adhesion after treatment with beta-glucan and chitosan. Conversely, xylooligosaccharides- and galacto-oligosaccharides-treated bacteria showed enhanced ability to adhere to enterocyte-like cell lines. Treatment with inulin did not show pronounced effect on lactobacilli adhesion. Overall, the three tested Lactobacillus strains displayed similar responses to the prebiotic treatment. Only pectin induced strain specific effect – reduced adhesion of L. rhamnosus 1010 and L. acidophilus 11 while the adhesion properties of L. paracasei 8458 were not significantly affected. These findings suggest that different types of prebiotic substances induce different mechanisms that modulate the adhesion ability and probably the probiotic activity of lactobacilli.


Keywords: Adhesion, probiotic, prebiotic, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus acidophilus.
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How to cite this article:

Pavlina Sredkova, Ilia Iliev, Balik Dzhambazov and Tsvetelina Batsalova. 2017. Prebiotic Treatment Influence the Adhesion Properties of Three Lactobacillus strains.Int.J.Curr.Microbiol.App.Sci. 6(9): 2914-2924. doi: https://doi.org/10.20546/ijcmas.2017.609.358