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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3682-3697
DOI: https://doi.org/10.20546/ijcmas.2017.608.445


Development of Cell Disruption Strategy for Enhanced Release of Intracellular Xylose Reductase from Pseudomonas putida BSX-46
Yogita Lugani and Balwinder Singh Sooch*
Enzyme Biotechnology Laboratory, Department of Biotechnology, Punjabi University,Patiala- 147002, Punjab, India
*Corresponding author
Abstract:

Xylose reductase (XR) is responsible for biotransformation of xylose to xylitol and it has gained significant position amongst industrial enzymes due to its role in meeting the demand of xylitol by utilizing agriculture waste. Xylitol is a rare pentose sugar alcohol having a number of therapeutic and pharmaceutical applications. There is a paradigm shift for biotechnological production of xylitol over conventional chemical method. Amongst biotechnological methods, enzymatic method is efficient over whole cell method for industrial xylitol production due to increased product yield and easy recovery of purified product. But, an efficient cell disruption strategy is critically required for the efficient recovery of intracellular XR from the microbial cell. The focus of present study is to test various physical, chemical and enzymatic methods for the disruption of cells of novel isolated Pseudomonas putida BSX-46 for maximum release of xylose reductase. Amongst all the methods adopted, sonication treatment given to cells pretreated with EDTA and β-mercaptoethanol was found to be most effective for maximum release of XR with an activity of 48.70±0.05 IU/mg of cells. The findings from the present study can result in development of an efficient method for making use of XR for industrial production of xylitol.


Keywords: Xylose reductase, Xylitol, Pseudomonas putida, Cell disruption, Sonication.
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How to cite this article:

Yogita Lugani and Balwinder Singh Sooch. 2017. Development of Cell Disruption Strategy for Enhanced Release of Intracellular Xylose Reductase from Pseudomonas putida BSX-46.Int.J.Curr.Microbiol.App.Sci. 6(8): 3682-3697. doi: https://doi.org/10.20546/ijcmas.2017.608.445