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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3584-3590
DOI: https://doi.org/10.20546/ijcmas.2017.608.430


Isolation and Identification of Bacteria from under Fingernails
Mohsen Hashim Risan*
College of Biotechnology/Al-Nahrain University, Baghdad, Iraq
*Corresponding author
Abstract:

This study was conducted to isolate and identify bacteria contaminants of under nails (long nails). The samples were collected randomly from 20 samples under nails (belonging to volunteers for sampling). This study was conducted between October to December, 2016 at College of Biotechnology, Al-Nahrain University. The isolated colonies were then sub cultured in nutrient agar and slants in order to obtain pure culture of all colonies. All students (100%) found to harbour bacteria on their under nails. Bacterial pathogens isolated from the under nails of students include Staphylococcus aureus (5 isolates), Bacillus cereus (2 isolates), Acinetobacter spp (1 isolates), Bacillus spp (7 isolates), Streptococcus spp (1 isolates), Pseudomonas aeruginosa (6 isolates), Klebsiella spp (2 isolates) and Unknown (2 isolates). Highest contamination of Bacillus spp was isolated. Showed Percentage of bacterial isolates from the samples collected from under nails after calculating the total percentage of each isolate Staphylococcus aureus 19.23%, Bacillus cereus 7.69%, Acinetobacter spp 3.84%, Bacillus spp 26.92%, Streptococcus spp 3.84%, Pseudomonas aeruginosa 23.07%, Klebsiella spp 7.69% and Unknown 7.69%. The highest prevalence were in male were (16 isolates) and were percentage of bacteria isolated 61.53% while in female were (10 isolates) and percentage of bacteria isolated 38.46%. Also showed results Percentage of total bacteria isolated of female and male, were 38.46% and 61.53 % respectively.


Keywords: Bacteria, Contaminants, Under nails.
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How to cite this article:

Mohsen Hashim Risan. 2017. Isolation and Identification of Bacteria from under Fingernails.Int.J.Curr.Microbiol.App.Sci. 6(8): 3584-3590. doi: https://doi.org/10.20546/ijcmas.2017.608.430