National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study was conducted to isolate and identify bacteria contaminants of under nails (long nails). The samples were collected randomly from 20 samples under nails (belonging to volunteers for sampling). This study was conducted between October to December, 2016 at College of Biotechnology, Al-Nahrain University. The isolated colonies were then sub cultured in nutrient agar and slants in order to obtain pure culture of all colonies. All students (100%) found to harbour bacteria on their under nails. Bacterial pathogens isolated from the under nails of students include Staphylococcus aureus (5 isolates), Bacillus cereus (2 isolates), Acinetobacter spp (1 isolates), Bacillus spp (7 isolates), Streptococcus spp (1 isolates), Pseudomonas aeruginosa (6 isolates), Klebsiella spp (2 isolates) and Unknown (2 isolates). Highest contamination of Bacillus spp was isolated. Showed Percentage of bacterial isolates from the samples collected from under nails after calculating the total percentage of each isolate Staphylococcus aureus 19.23%, Bacillus cereus 7.69%, Acinetobacter spp 3.84%, Bacillus spp 26.92%, Streptococcus spp 3.84%, Pseudomonas aeruginosa 23.07%, Klebsiella spp 7.69% and Unknown 7.69%. The highest prevalence were in male were (16 isolates) and were percentage of bacteria isolated 61.53% while in female were (10 isolates) and percentage of bacteria isolated 38.46%. Also showed results Percentage of total bacteria isolated of female and male, were 38.46% and 61.53 % respectively.
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