Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2524-2541
DOI: https://doi.org/10.20546/ijcmas.2017.608.300


Phylogenetic and Antimicrobial Characteristics of a Novel Streptomyces sp. Ru87 Isolated from Egyptian Soil
 
Dina H. Amin1*, Sahar Tolba1, Assem Abolmaaty2,Nagwa A. Abdallah1 and Elizabeth M.H. Wellington3
1Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
2Department of Food Science, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
3School of life Sciences, University of Warwick, Coventry, United Kingdom
*Corresponding author
Abstract:

A novel Streptomyces sp. Ru87 was isolated from Egyptian soil and showed significant inhibition activities against a number of food and blood borne pathogens. Morphological and chemotaxonomic characteristics showed that Ru87 belongs to genus Streptomyces. 16 S rRNA gene sequence (KY775360), showed 98% identity with Streptomyces cacaoi strain NBRC 12748. However, phylogenetic analysis indicated that it is distinct from the known species. A cultured extract of this strain inhibits the growth of Staphylococcus aureus and some other foodborne and blood borne pathogens. The highest yield of antimicrobial agent was obtained after 6 days of culturing 4 x 105 CFU/ml in starch casein broth media with agitation (150 RPM) at 30oC, pH 7.0. The analytical studies using Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) illustrated that the final extract represent one pure component. The antimicrobial activity of bioactive fraction was investigated using minimum inhibitory concentrations (MIC) and reporting 24 µg active of fraction/ml against S. aureus. Partial characterization was conducted using Mass spectroscopy, IR and 1H, 13C Nuclear Magnetic Resonance (NMR) indicated for the first time that Streptomyces sp. Ru87 produce antibiotics related to aromatic peptides.


Keywords: Actinomycetes, Staphylococcus aureus, Aromatic peptides antibiotics, Foodborne pathogens, Phylogenetic analysis, 16S rRNA gene sequencing.
Download this article as Download

How to cite this article:

Dina H. Amin, Sahar Tolba, Assem Abolmaaty, Nagwa A. Abdallah and Elizabeth, Wellington, M.H. 2017. Phylogenetic and Antimicrobial Characteristics of a Novel Streptomyces sp. Ru87 Isolated from Egyptian Soil.Int.J.Curr.Microbiol.App.Sci. 6(8): 2524-2541. doi: https://doi.org/10.20546/ijcmas.2017.608.300