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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2392-2400
DOI: https://doi.org/10.20546/ijcmas.2017.608.283


Bioavailability of Albendazole and its Metabolities in Plasma of Pangasianodon hypophthalmus with High Performance Liquid Chromatography
Showkat Ahmad Dar1*, Vipin Nautiyal1, Vikas Phulia2, Subodh Gupta1, Primal Sardar1 and N.P. Sahu2
1Division of Fish Nutrition, Physiology, and Biochemistry, Central Institute  of Fisheries Education, Mumbai- 400061, India
2GADVASU Ludhiana, Punjab-141004, India
*Corresponding author
Abstract:

In the present experiment the depletion profile study of Albendazole and its metabolites (ABZSO and ABZSO2) were carried out in the plasma of Pangasianodon hypophthalmus. The experiment consists of three treatment groups and control. A 20mg/kg body weight dose of ABZ was given through intraperitoneal, intramuscular and orally respectively to the three treatment groups while no drug was given to control group. The blood samples were collected at 0.5, 1, 2, 4, 8, 12, 24, and 48h intervals to determine the ABZ, ABZSO and ABZSO2 using High Performance Liquid Chromatography (HPLC) having C-18 reversed–phase analytical column. The samples were processed by mobile phase of Methanol-Water (60:40) at a constant flow rate of 1 ml/ min and detected at 295 nm using a UV/Visible detector. The drug was detected in the plasma of orally given treatment group only. The Maximum concentration (Cmax) of ABZ 3.461± 0.06 μgml-1 found at 8 h (Tmax) and retention time (RT) of 23.88 minutes. For ABZSO Maximum concentration (Cmax) detected was 0.037 ±0.009 μgml-1 at 8 h (Tmax) with RT of 5.61minutes and ABZSO2 Cmax found was 0.023 ± 0.007 μgml-1 at 12 h (Tmax) with RT of 19.4 minutes. The concentration of metabolites reached to the levels of negligible after 48 h. The present study reveals that giving ABZ through orally is efficient method for treatment of helminth infections in fishes.


Keywords: Albendazole, HPLC, Pangasianodon-hypophthalmus, Maximum concentration (Cmax), Retention Time (RT).
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How to cite this article:

Showkat Ahmad Dar, Vipin Nautiyal, Vikas Phulia, Subodh Gupta, Primal Sardar and Sahu, N.P. 2017. Bioavailability of Albendazole and its Metabolities in Plasma of Pangasianodon hypophthalmus with High Performance Liquid Chromatography.Int.J.Curr.Microbiol.App.Sci. 6(8): 2392-2400. doi: https://doi.org/10.20546/ijcmas.2017.608.283