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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2109-2119
DOI: https://doi.org/10.20546/ijcmas.2017.608.251


A Study on Colocasia as a Suitable Substrate for Production of Cellulase
Gayatree Jadeja1 and Shilpi Verma2*
1KVK Arnej, Anand Agriculture University, Gujarat, India
2KVK Neemuch, RVSKVV, Gwalior, India
*Corresponding author
Abstract:

The study was planned to fulfill the objectives like to isolate the cellulolytic bacteria and fungi, determination of the cellulase production from the colocasia as a substrate using the isolated cellulolytic fungi and determination of the optimal condition for the cellulase production condition such as temperature (30ËšC, 35ËšC, 40ËšC, 45ËšC and 50ËšC), pH (Alkaline and Acidic), Time (24, 48, 72 hrs) and substrate (Colocasia Leaf, Root, Stem) and estimate CMCase activity, reducing sugar and protein. The cellulase biomass was also measured on dry weight base. This study revealed that colocasia leaf, stem and root which are very economic as well as produce large amount of cellulase enzyme when hydrolysed by cellulytic fungi in acidic pH. The suitable conditions for cellulase production are 50°C temperature and 24 hrs, 48 hrs and 72 hrs are suitable for stem, root and leaf, respectively. So instead of being left behind for natural degradation, this can be utilized effectively under these conditions, to produce cellulase.


Keywords: Cellulase, Colocasia, Cellulolytic fungi, Cellulolytic bacteria, Submerge fermentation, Cellulase biomass.
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How to cite this article:

Gayatree Jadeja and Shilpi Verma. 2017. A Study on Colocasia as a Suitable Substrate for Production of Cellulase.Int.J.Curr.Microbiol.App.Sci. 6(8): 2109-2119. doi: https://doi.org/10.20546/ijcmas.2017.608.251