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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3176-3180
DOI: https://doi.org/10.20546/ijcmas.2017.608.378


A New Agar Plate Assisted Slide Culture Technique to Study Mycoparasitism of Trichoderma sp. on Rhizoctonia solani and Fusarium oxysporium
K. A. Bhat*
Division of plant Protection, Faculty of Agriculture Wadura, Sher-i-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
*Corresponding author
Abstract:

Mycoparasitism by Trichoderma sp. is one of the important modes of action exhibited by biocontrol agents against host fungi including plant pathogenic ones. Its study in-vitro constitutes considerable importance while studying bio-efficacy of an antagonist against a fungal pathogen. At present there is no standard, rapid, easy and reliable method to study mycelial interaction between a possible mycoparasitic fungi and host. An easy, cheap, and rapid agar plate assisted slide culture technique is developed which facilitates the study of in-vitro mycoparasitism with ease. With the help of this technique mycoparasitism by a strain of Trichoderma sp. was studied against pathogenic Rhizoctonia solani and Fusarium oxysporium. A drastic mycoparasitism in the form of coiling and tightly sticking with occasional formation of appressoria like structures was observed while studying mycoparasitism with the help of this method.


Keywords: Mycoparasitism, in-vitro, Technique, Trichoderma, Plant pathogens
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How to cite this article:

Bhat, K.A. 2017. A New Agar Plate Assisted Slide Culture Technique to Study Mycoparasitism of Trichoderma sp. o on Rhizoctonia solani and Fusarium oxysporiumInt.J.Curr.Microbiol.App.Sci. 6(8): 3176-3180. doi: https://doi.org/10.20546/ijcmas.2017.608.378