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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:8, August, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3176-3180
DOI: https://doi.org/10.20546/ijcmas.2017.608.378


A New Agar Plate Assisted Slide Culture Technique to Study Mycoparasitism of Trichoderma sp. on Rhizoctonia solani and Fusarium oxysporium
K. A. Bhat*
Division of plant Protection, Faculty of Agriculture Wadura, Sher-i-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
*Corresponding author
Abstract:

Mycoparasitism by Trichoderma sp. is one of the important modes of action exhibited by biocontrol agents against host fungi including plant pathogenic ones. Its study in-vitro constitutes considerable importance while studying bio-efficacy of an antagonist against a fungal pathogen. At present there is no standard, rapid, easy and reliable method to study mycelial interaction between a possible mycoparasitic fungi and host. An easy, cheap, and rapid agar plate assisted slide culture technique is developed which facilitates the study of in-vitro mycoparasitism with ease. With the help of this technique mycoparasitism by a strain of Trichoderma sp. was studied against pathogenic Rhizoctonia solani and Fusarium oxysporium. A drastic mycoparasitism in the form of coiling and tightly sticking with occasional formation of appressoria like structures was observed while studying mycoparasitism with the help of this method.


Keywords: Mycoparasitism, in-vitro, Technique, Trichoderma, Plant pathogens

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How to cite this article:

Bhat, K.A. 2017. A New Agar Plate Assisted Slide Culture Technique to Study Mycoparasitism of Trichoderma sp. o on Rhizoctonia solani and Fusarium oxysporiumInt.J.Curr.Microbiol.App.Sci. 6(8): 3176-3180. doi: https://doi.org/10.20546/ijcmas.2017.608.378
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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