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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2625-2635
DOI: https://doi.org/10.20546/ijcmas.2017.608.312


Detection of Intimin Gene and Its Variants, In E. coli Isolates from Calf Diarrhoea Cases
Vandana Gupta1*, Ashish Roy2 and Megha Katare3
1Department of Veterinary Microbiology, College of Veterinary Science & A.H.,Rewa, M.P., India
2Department of Veterinary Microbiology, College of Veterinary Science & A.H.,Anand, Gujarat, India
3Department of Veterinary Pathology, College of Veterinary Science & A.H.,Anjora, Chhatisgarh, India
*Corresponding author
Abstract:

The present study was designed to study the presence of intimin gene and its variants in 91 E. coli isolates. All 91 E. coli isolates were screened for the presence of intimin gene by PCR, among which only 15 isolate (16.8 percent) were positive for eaeA gene. Out of 15 isolates positive for eae (intimin) were assayed for intimin variants viz., eae1(811bp), eae1(804bp), eae2(808bp), eae (833bp), eae (206bp), and eae (468bp) by PCR. Eight isolates could be typed using 6 intimin variant primer set and 2 isolate (13.3percent) were positive for eae1 variant, one isolate (6.67percent) was positive for eae1 and five (33.3percent) were positive for eae2. Intimin gene codes for different types of protein which are immunogenic in nature and thus it can represent an important tool for E. coli typing in routine diagnostic as well as epidemiological and clonal studies. Different intimin variants are responsible for different host tissue cell tropism which could be taken into consideration to identify the specific intimin variant types responsible for attachment to specific tissue tropism.


Keywords: E. coli, Intimin, Intimin gene variants.
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How to cite this article:

Vandana Gupta, Ashish Roy and Megha Katare. 2017. Detection of Intimin Gene and Its Variants, In E. coli Isolates from Calf Diarrhoea Cases.Int.J.Curr.Microbiol.App.Sci. 6(8): 2625-2635. doi: https://doi.org/10.20546/ijcmas.2017.608.312