National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was designed to study the presence of intimin gene and its variants in 91 E. coli isolates. All 91 E. coli isolates were screened for the presence of intimin gene by PCR, among which only 15 isolate (16.8 percent) were positive for eaeA gene. Out of 15 isolates positive for eae (intimin) were assayed for intimin variants viz., eaeï¢1(811bp), eaeï§1(804bp), eaeï§2(808bp), eaeï¤ (833bp), eaeïº (206bp), and eaeï¸ (468bp) by PCR. Eight isolates could be typed using 6 intimin variant primer set and 2 isolate (13.3percent) were positive for eaeï¢1 variant, one isolate (6.67percent) was positive for eaeï§1 and five (33.3percent) were positive for eaeï§2. Intimin gene codes for different types of protein which are immunogenic in nature and thus it can represent an important tool for E. coli typing in routine diagnostic as well as epidemiological and clonal studies. Different intimin variants are responsible for different host tissue cell tropism which could be taken into consideration to identify the specific intimin variant types responsible for attachment to specific tissue tropism.
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