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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 596-607
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.068


In silico Analysis of Physicochemical Properties of Hyperthermophillic and Thermophillic Nitrile Hydratases
Pawan Kumar Sharma1* and Tek Chand Bhalla2
Department of Agricultural Biotechnology, CSKHPKV Palampur HP 176062, India
Department of Biotechnology Himachla Pradesh University, Shimla HP171005, India
*Corresponding author
Abstract:

Nitrile hydratases (NHases, EC 4.2.1.84) are non-heme iron-containing or non-corrinoid cobalt-containing enzymes that catalyze the hydration of nitriles to their corresponding amides. Present research a computational study with statistical correlation has been done using the sequences of thermophiles and hyper thermophiles. The number of amino acids were found in range of 249 to 288 for hyperthermophiles and 235 to 292 in thermophiles, molecular weight range was 26738.7 to 32032.4 and 26044.9 to 35529.7, theoretical pI estimated were 6.18 to 9.44 and 6.16 to 9.68, negatively charged residues were 28 to 41 in both cases, positively charged residues were 30 to 39 and 30 to 46, extinction coefficient estimated were 8940 to 37930 and 17880 to 40910, aliphatic index observed as 101.73 to 111.74 and 90.49 to 113.01, instability index values were 38.68 to 40.89 and 30.71 to 42.11, GRAVY values were positive and negative for hyperthermophiles and thermophiles, respectively. Statistical correlation was found among the cysteine as 5.8840 , Met (M)   6.9980 , Phe (F) 9.5972 , Thr (T) 5.3864 , Tyr (Y)   7.7134 . This shows sulphur containing, aromatic and bulky groups as significant amino acids.


Keywords: Nitrile hydratase, amino acids, Molecular weight, ProtParam EXPASy, Theoretical pI, instability index, Hydropathicity.
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How to cite this article:

Pawan Kumar Sharma and Tek Chand Bhalla. 2016. In silico Analysis of Physicochemical Properties of Hyperthermophillic and Thermophillic Nitrile Hydratases.Int.J.Curr.Microbiol.App.Sci. 5(4): 596-607. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.068