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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 833-897
DOI: https://doi.org/10.20546/ijcmas.2017.608.111


Characterization of ACC Deaminase producing B. cepacia, C. feurendii and S. marcescens for Plant Growth Promoting activity
A. Maxton1, P. Singh1, S.M. Prasad1, Aruna Andy2 and S.A. Masih3*
1Department of Molecular and Cellular Engineering
2Directorate of Research
3Centre for Transgenic Studies, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, U.P. India, 211007
*Corresponding author
Abstract:

Finding solutions to global issues like food security entails greater use of chemicals fertilizers, pesticides, fungicides etc. posing serious risks of soil salinity, heavy metal bioaccumulation. PGPR produce enzyme ACC deaminase which degrades (ACC) 1-aminocyclopropane-1-carobylic acid the immediate precursor of the plant growth hormone ethylene, into α-ketobutyrate and ammonia, thus lowering the level of ethylene in a developing or stressed plant. In the present study, three rhizobacterial strains were isolated from rhizospheric soil of mustard plant in Allahabad region. On the basis of morphological, biochemical, molecular characterization, these isolates were identified as Burkholderia cepacia, Citrobacter feurendii I and Serratia marcescens. The 16S rRNA gene sequences of B. cepacia, and S. marcescens, C. feurendii were submitted to NCBI GenBank under accession numbers LC169488, LC169489, LC169490, respectively, followed by their phylogenetic analysis predicting significant sequence homology with related sequences of NCBI Gene bank. After isolation ACC Deaminase enzyme was partially purified and molecular weight was determined 35-42 kDa. Screeningthe rhizobacterial isolates for plant growth promoting traits (ACC deaminase activity assay; Ninhydrin assay; Phosphate solubilization assay; Production of Siderophore, IAA, Gibberellic Acid, HCN, Ammonia) revealed thatS. marcescens, C. feurendii and B. cepacia are capable of producing ACC deaminase and solubilizing phosphate along with exhibiting various other plant promoting traits.


Keywords: PGPR characterization, ACC deaminase, Ninhydrin assay, Plant growth.
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How to cite this article:

Maxton, A., P. Singh, S.M. Prasad, Aruna Andy and Masih, S.A. 2017. Characterization of ACC Deaminase Producing B. cepacia; C. feurendii and S. marcescens for Plant Growth Promoting activity.Int.J.Curr.Microbiol.App.Sci. 6(8): 833-897. doi: https://doi.org/10.20546/ijcmas.2017.608.111