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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 836-842
DOI: https://doi.org/10.20546/ijcmas.2017.608.106


Surface Sterilization for Reducing Microbial Contamination in In Vitro Propagation of Lasora (Cordia myxa Roxb.) Using Nodal Segments
Minakshi Padhi* and S.P. Singh
Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India
*Corresponding author
Abstract:

Lasora (Cordia myxa Roxb.) is commonly grown as minor or underexploited crop having great medicinal and food value which belongs to the family Boraginaceae, originates along the Himalayan tract upto 1,500 metres. In-vitro technique is accepted widely to produce virus free plants in a very large number. Production of virus free planting material using meristem culture has been made possible in this crop. An experiment entitled “Surface sterilization for reducing microbial contamination in In-vitro propagation of lasora (Cordia myxa Roxb.) using nodal segments” was conducted at Institute of Agricultural Sciences, Banaras Hindu University, Varanasi during 2015-16 which aims to elucidate standardization of sterilization process through various concentration of HgCl2 at 0.1% and NaOCl at 1% concentration followed by 70% ethanol in lasora. The effect of various exposure time of HgCl2 and NaOCl on different explants was studied and found to be best when explants treated with HgCl2 (0.1%) for 10 minutes, resulted 85.50 per cent aseptic culture and 83.25 per cent survival of explants. Whereas, maximum aseptic culture was found with 1% sodium hypochloride when treated for 21 minutes followed by 70% ethanol for 30 sec This can be explained by the fact that requirements for surface sterilization are different and depend on the tissue type and the nature of the explants used for in-vitro propagation.


Keywords: Aseptic, Sterilization, Explants, Mercuric chloride, Sodium hypochloride.
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How to cite this article:

Minakshi Padhi and Singh, S.P. 2017. Surface Sterilization for Reducing Microbial Contamination in In Vitro Propagation of Lasora (Cordia myxa Roxb.) Using Nodal Segments.Int.J.Curr.Microbiol.App.Sci. 6(8): 836-842. doi: https://doi.org/10.20546/ijcmas.2017.608.106