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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 753-761
DOI: https://doi.org/10.20546/ijcmas.2017.608.096


Isolation and Screening of Bacterial and Fungal Isolates for Plant Growth Promoting Properties from Tomato (Lycopersicon esculentum Mill.)
M. Rajendra Prasad*, B. Vidya Sagar, G. Uma Devi, S. Triveni, S.R. Koteswar Rao and K. Damodara Chari
Department of Plant Pathology, College of Agriculture, PJTSAU Hyderabad, Telangana, India
*Corresponding author
Abstract:

In the present study About 24 isolates of fungal bio-control agent particularly Trichoderma spp and 12 bacterial isolates were isolated from the rhizosphere of tomato which was identified based on morphological and cultural characteristics. Among 24 isolates of Trichoderma spp ten and twelve bacterial isolates were identified with antagonistic ability and tested against the test soil borne pathogens P. debaryanum, R. solani and S. rolfsii. The Trichoderma harzianum-7 and Trichoderma harzianum-1 were found effective in inhibition of mycelium (80.03), (62.53 and 95.1) against P. debaryanum, R. solani and S. rolfsii respectively under in vitro conditions. The efficient isolates further screened for functional plant growth promoting properties. Among twelve bacterial isolates, Pseudomonas fluorescence-3 recorded highest solubilization zone followed by Pseudomonas fluorescence-2. All the bio-agents showed positive results to ammonia production except Trichoderma harzianum-6. Similarly all the bio-gents exhibited positive results for HCN production. Highest HCN production was observed with the Trichoderma viride-2, 9, 10 and Trichoderma harzianum-4, 7, 8, 13 and bacterial isolates of Bacillus subtilis-2, 4 and Pseudomonas fluorescence-10. IAA production was observed with Trichoderma but the highest IAA production was noticed with the bacterial isolates of Bacillus subtilis-5, Pseudomonas fluorescence-2 and 12. All the bacterial isolates showed siderophore production in the plate assay of which the bacterial isolates of Bacillus subtilis-4, Pseudomonas fluorescence-1 showed prominent orange colour zone. The Trichoderma viride 9 and Trichoderma harzianum-2 showed lower production of siderophores.


Keywords: Pythium debaryanum, Rhizoctonia solani, Sclerotium rolfsii, Trichoderma harzianum, Trichoderma viride, Pseudomonas fluorescence, Soil borne pathogens.
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How to cite this article:

Rajendra Prasad, M., B. Vidya Sagar, G. Uma Devi, S. Triveni, S.R. Koteswar Rao and Damodara Chari, K. 2017. Isolation and Screening of Bacterial and Fungal Isolates for Plant Growth Promoting Properties from Tomato (Lycopersicon esculentum Mill.).Int.J.Curr.Microbiol.App.Sci. 6(8): 753-761. doi: https://doi.org/10.20546/ijcmas.2017.608.096