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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 656-668
DOI: https://doi.org/10.20546/ijcmas.2017.608.083


Cross Legume Species/Genera Transferability of SSR Markers and their Utility in Assessing Polymorphism among Advanced Breeding Lines in Dolichos Bean (Lablab purpureus L.)
M.S. Shivakumar1*, S. Ramesh2, A. Mohan Rao2, H.R. Udaykumar2 and C.M. Keerthi2
1Indian Council of Agricultural Research-Indian Institute of Spices Research,Kozhikode, Karnataka, India
2Department of Genetics and Plant Breeding, University of Agricultural Sciences (UAS), Bengaluru 560 065, Karnataka, India
*Corresponding author
Abstract:

The use of transferable cross-species/genera SSR markers is an alternative strategy to ensure availability of markers in genomic resources-limited crops such as dolichos bean. A total of 275 cross legume species/genera SSR markers were examined for their transferability to dolichos bean. 126 of 275 cross legume species/genera SSR markers (45.81%) were found transferable to dolichos bean. Transferability varied with cross legume species/genera with 83.33% of Medicago truncatula, 78.57% of greengram, 77.77% of chickpea, 53.33% of soybean and 33.33% of cowpea SSR markers being transferable to dolichos bean. The extent transferability of SSR markers based on simple di-/tri-nucleotide repeat motifs was higher than those based on penta-/tetra-/complex nucleotide repeat motifs. Of the 126 transferable SSR markers, 64 produced amplification in all the 14 advanced breeding lines with an average of 2.42 alleles per SSR locus. Six of the 64 SSR markers were most informative with four allelic variants.


Keywords: Repeat motifs, Transferability, Legumes, Cladogram.
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How to cite this article:

Shivakumar, M.S., S. Ramesh, A. Mohan Rao, H.R. Udaykumar and Keerthi, C.M. 2017. Cross Legume Species/Genera Transferability of SSR Markers and their Utility in Assessing Polymorphism among Advanced Breeding Lines in Dolichos Bean (Lablab purpureus L.).Int.J.Curr.Microbiol.App.Sci. 6(8): 656-668. doi: https://doi.org/10.20546/ijcmas.2017.608.083