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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 286-294
DOI: https://doi.org/10.20546/ijcmas.2017.608.038


Production in Cell Biomass and Carotenoids under the Effect of a Saline Stress in Microalgae Dunaliella spp. Isolated from Moroccan Saharian Saline
I. Charioui1,2, M. Chikhaoui1*, F. El Filali3, M. Abbassi2,4, A. Banaoui2 and A. Kaaya4
1Laboratory of Biotechnology,
3Laboratory of Food Bacteriology, Specialized Center of Valorization and Technology of Marine Products, National Institute for Marine Research (INRH), Agadir 80090, Morocco
2Laboratory of the Aquatic Systems: Marine and Continental Environment, Faculty of Science, University IbnZohr, Agadir 8006, Morocco
4Bio-environment, Health and Bioressources Team, Department of Biology, Faculty of Science, Agadir 80060, Morocco
*Corresponding author
Abstract:

Microalgae contain a battery of bioactive molecules such as carotenoids used in various industrial sectors. This is the case of the microalga Dunaliella spp., whose cells, under stress conditions, accumulate remarkable levels of carotenoids. In this study, three native strains of Dunaliella (D. viridis (DUN4), D. salina (DUN5) and D. salina (DUN6)) isolated from the Moroccan saharian saline on the Atlantic coast were subjected to different salinity regimes (0.5, 1.5, 2.5, 3.5 and 4.5 M NaCl) to evaluate the ability to produce biomass and carotenoid biosynthesis in this species. Total carotenoids were determined by spectrophotometry. The strains are cultured for 15 days on F/2 Guillard medium and aerated at 25±1°C. Strain DUN5 showed, in the presence of 2.5 M NaCl, a maximum of carotenoids of the order of 3.267μg.ml-1, 3,206μg.ml-1 in the strain DUN 4 to 3.5 M NaCl, and 2,314μg.ml-1 in the strain DUN 6 at 0.5 M NaCl. The highest cell densities are recorded in the strain DUN 4 being of the order of 3.199.106 Cells.ml-1, the concentrations remains lower in the strains DUN5 and DUN6. These results indicate that each strain has a different behavior and physiological response to salt stress. Moreover, these microalgae have remarkable capacities and can constitute a potential source of biochemical molecules of industrial interest.


Keywords: Salinity, Biosynthesis, Carotenoids, Dunaliella spp., Microalgae.
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How to cite this article:

Charioui, I., M. Chikhaoui, F. El Filali, M. Abbassi, A. Banaoui and Kaaya, A. 2017. Production in Cell Biomass and Carotenoids under the Effect of a Saline Stress in Microalgae Dunaliella Spp. Isolated from Moroccan Saharian Saline.Int.J.Curr.Microbiol.App.Sci. 6(8): 286-294. doi: https://doi.org/10.20546/ijcmas.2017.608.038