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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4549-4556
DOI: https://doi.org/10.20546/ijcmas.2017.607.475


Molecular Study of Efflux Genes in Pseudomonas aeruginosa Isolated from Clinical Samples
Maysaa El Sayed Zaki1, Ahmed Elewa1 and Nashwa M. Al-Kasaby2*
1Clinical Pathology department Faculty of Medicine, Mansoura University, Egypt
2Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt
*Corresponding author
Abstract:

Pseudomonas aeruginosa is a common nosocomial pathogen associated with several clinical infections. It shows high resistance to most antibiotics classes due to multiple antibiotic resistance mechanisms. The aim of the present study was to assess the presence of efflux system genes by multiplex PCR among clinical isolates of P. aeruginosa. The study was conducted on clinical isolates of P. aeruginosa collected from Mansoura University hospital from January 2016 to December 2016. Identification of P. aeruginosa was performed according to standard microbiological techniques and antibiotics susceptibility was determined by disc diffusion methods. Multiplex PCR was used for amplification of MexA, MexB, and MexR genes. For detection of OprM and OprD genes, two separate rounds of PCR were performed. Isolated P. aeruginosa showed high resistance to gentamicin (90.6%), amikacin (66.03%) ceftazidime (77.4%), Aztreonam (64.2%), imipenem (47.2%) and ciprofloxacin (26.4%).PCR study for efflux genes revealed the presence of genes in 66.03% of P. aeruginosa isolates; combined MexB and OprD in 60.4%, MexB alone, and combined MexB and OprD in 1.9% for each. The presence of efflux genes was associated significantly with high resistance to gentamicin, ceftazidime, Cefepime, Aztreonam, Ciprofloxacin, Amikacin, Meropenem and Imipenem (P value 0.0001, 0.003, 0.0001, 0.008, 0.02, 0.03, 0.0001 and 0.0001; respectively). The present study highlights the importance of genes controlling efflux pumps as an important cause associated with P. aeruginosa resistance to antibiotics. Longitudinal large scales studies are required for further analysis of these genes and its expression effects on antibiotics resistance of P. aeruginosa.


Keywords: Multiplex PCR, Pseudomonas aeruginosa, Efflux genes
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How to cite this article:

Maysaa El Sayed Zaki, Ahmed Elewa and Nashwa M. Al-Kasaby. 2017. Molecular Study of Efflux Genes in Pseudomonas aeruginosa Isolated from Clinical Samples.Int.J.Curr.Microbiol.App.Sci. 6(7): 4549-4556. doi: https://doi.org/10.20546/ijcmas.2017.607.475