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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4521-4531
DOI: https://doi.org/10.20546/ijcmas.2017.607.471


Process Parameter Optimization for Asparaginase Production from O. intermedium: A Potential Candidate for Acrylamide Mitigation
Anjana Sharma* and Shubhi Mishra
Bacteriology Laboratory, Department of P. G. Studies and Research in Biological Science, Rani Durgavati University, Pachpedi, Jabalpur 482001, Madhya Pradesh, India
*Corresponding author
Abstract:

Asparaginase (asn) is a promising candidate for the obviation of acrylamide formation in baked and fried food stuffs by pre amidohydolase of free asparagine present. In this study optimized the medium composition for asparaginase production from Ochrobactrum. intermedium using one variable at a time approach. Maximum yield of asparaginase was recorded with batch time 18 h, inoculum size 2%, pH 7.0, and temperature 40˚C. Lactose, yeast extract, magnesium ions, asparagine were found to be best carbon, nitrogen, mineral ions and inducer respectively for asparaginase production. An overall 5.045 (13.956±0.07 IU/ ml) fold increased as compared to initial un optimized activity of 2.766 IU/ml was obtained. These results suggested that selected parameter can be promisingly enhanced the asparaginase production. The further attempts are underway for validation of significant factors by statistical approach to influence asparaginase yield from the strain for acrylamide inhibition.


Keywords: Process, Parameter, Optimization and O. intermedium.
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How to cite this article:

Anjana Sharma and Shubhi Mishra. 2017. Process Parameter Optimization for Asparaginase Production from O. intermedium: A Potential Candidate for Acrylamide Mitigation.Int.J.Curr.Microbiol.App.Sci. 6(7): 4521-4531. doi: https://doi.org/10.20546/ijcmas.2017.607.471