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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4379-4386
DOI: https://doi.org/10.20546/ijcmas.2017.607.456


Sperm Mediated Gene Transfer (SMGT) in Chicken
Sushil Kumar*, Govind Mohan, Revanasiddu Deginal, Saleem Yousuf, N. Anand Kumar and P. Sateesh Kumar
Ph.D. Scholar, ICAR-National Dairy Research Institute, Karnal, Haryana-132001, India
*Corresponding author
Abstract:

Sperm-mediated gene transfer (SMGT) is a transgenic technique involves transfer of the genes based on the spermability to bind spontaneously and internalize the exogenous DNA, and transport it into an oocyte during fertilization to produce genetically modified animals. Spermatozoa play a major role in transgenesis in virtually all species. Their ability to take up exogenous DNA molecules to transmit novel genetic information to the offspring after fertilization can be exploited. The assisted techniques like electroporation, lipofection, Restriction Enzyme Mediated Integration (REMI), Intracytoplasmic Sperm injection (ICSI) and linker based SMGT have been developed to increase the DNA uptake by the spermatozoa. SMGT can be regarded as a potential source of mutation for the host genome. Avian species, particularly chickens, have been increasingly used in transgenic research due to their inherent advantages, such as short generation times, high semen production and potential applications as transgenic bioreactors for heterologous protein production. The application of transgenic technology to domestic poultry offers an alternative to conventional practices for the improvement of this highly productive agricultural species and its use as a bioreactor model.


Keywords: Sperm Mediated Gene Transfer, oocyte.
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How to cite this article:

Sushil Kumar, Govind Mohan, Revanasiddu Deginal, Saleem Yousuf, N. Anand Kumar and Sateesh Kumar, P. 2017. Sperm Mediated Gene Transfer (SMGT) in Chicken.Int.J.Curr.Microbiol.App.Sci. 6(7): 4379-4386. doi: https://doi.org/10.20546/ijcmas.2017.607.456