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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 405-412
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.048


Cloning, Expression and Comparative Study of White Spot Syndrome Virus (WSSV) Thymidine Kinase Gene during Infection in Penaeus monodon
R. Vaishnavi, Amrendra Kumar and A. Saravanakumar*
Center of advance studies in Marine biology, Faculty of Marine Sciences, Annamalai University, Paranagipettai, Tamilnadu, India, 600502
*Corresponding author
Abstract:

Thymidine kinase (EC 2.7.1.21) is the key enzyme in the pyrimidine salvage pathway catalyzing the transfer of the phosphate from ATP to thymidine to produce dTMP. Several analogue of viral TK has been studied for inhibiting the function of TK in human viruses, but there is no any analogue for WSSV TK. Hence this study has undertaken to derive the mechanism of WSSV TK structurally based functions, in this Study Total RNA was isolated, WSSV TK gene was amplified from synthesized C-DNA, initially TK gene was cloned in PTZ T tail vector, subcloned I pRSET-B vector and expressed for further study, purified product from pRSET-B vector was sequenced and translated, blast result shown the match from Leishmania donovani TK protein. Furthermore physio-chemical parameter of WSSV TK protein was analyzed, and active sites in 3Dstructure of WSSV TK protein was studied, which will lead towards to design small inhibitory analogue of WSSV TK.


Keywords: White spot syndrome, Shrimp, Thymidine kinase, DNA replication, Penaeus monodon.
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How to cite this article:

Vaishnavi, R., Amrendra Kumar and Saravanakumar, A. 2016. Cloning, Expression and Comparative Study of White Spot Syndrome Virus (WSSV) Thymidine Kinase Gene during Infection in Penaeus monodon.Int.J.Curr.Microbiol.App.Sci. 5(4): 405-412. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.048