Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2069-2076
DOI: https://doi.org/10.20546/ijcmas.2017.607.244


Genetic Diversity Analysis in Sunflower (Helianthus annuus L.)parental Lines Using SSR and RAPD Markers
P.G. Suresha1, Vikas V. Kulkarni2, S.M. Supriya1, S. Darshan3* and Chandrashekar B. Patil1
1Department of Genetics and Plant Breeding, College of Agriculture, Raichur,Karnataka, India -584 104
2AICRP on Sunflower, MARS, University of Agricultural Sciences, Raichur,Karnataka, India-584 104
3Department of Plant Breeding and Genetics, College of Agriculture, Vellayani,Kerala, India-695 522
*Corresponding author
Abstract:

Using molecular markers one can examine genetic diversity of species, cultivars, lines and forms of sunflower, identify genotypes, systematize collection material, classify heterosis groups and construct genetic maps. Fourteen parental genotypes of sunflower viz.,CMS-2A, CMS-821A, CMS-850A, R-10-46-2A, CMS-A4, CMS-A6, CMS-A10, R-GM-39, R-GM-41, R-GM-49, R-GM-69, 83-Br, R-393 and R2F01120B were analyzed for genetic diversity using 15 RAPD and 44 SSR primers. The genetic similarity index computed considering pooled data of SSR and RAPD markers showed very wide range from 0.18 to 0.74 indicating high variability among 14 sunflower genotypes. The dendrogram separated 14 sunflower genotypes into four main clusters. The first cluster A having genetic similarity index of 0.64 included three sunflower genotypes (CMS 2A,CMS 821 A and CMS A4) indicating the narrow genetic base, however cluster B showed sufficient genetic diversity among four sunflower genotypes R-GM-49, R-GM-69, R-GM-41 and R-393 as these restorer lines have been derived from different genetic background.


Keywords: Sunflower, Diversity analysis, SSR, RAPD.
Download this article as Download

How to cite this article:

Suresha, P.G., Vikas V. Kulkarni, S.M. Supriya, S. Darshan and Chandrashekar B. Patil. 2017. Genetic Diversity Analysis in Sunflower (Helianthus annuus L.) Parental Lines Using SSR and RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 6(7): 2069-2076. doi: https://doi.org/10.20546/ijcmas.2017.607.244