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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 1835-1857
DOI: https://doi.org/10.20546/ijcmas.2017.607.221


Molecular Characterization and Stability Analysis for Yield and its Components Traits in Soybean (Glycine max L.)
A.A. El-Mouhamady1*, H.M.Abdel-Rahman1, T.A. Elewa2 and K.A. Aboud1
1Genetics and Cytology Department, Genetic Engineering and Biotechnology Division,  National Research Centre, 33 El Buhouth ST., 12622, Dokki, Giza, Cairo, Egypt
2Field crops Research Dept., Agriculture and Biological Division, National Research Center, Dokki, Giza, Egypt
*Corresponding author
Abstract:

Seven soybean entries namely;(Crawford, Giza 21, Giza 22, Giza 35, Giza 82, Giza 83 and Giza 111) with different reaction for highly yielding, tolerance for salinity, water deficit conditions and resistance for diseases were evaluated under twelve environments through two years (2015, 2016 seasons), three locations (the farm of agricultural research in Mansoura city, Dakahlia Governorate, the farm of agriculture research center, Sakha station Kafr El-Sheikh governorate and the farm of national research Centre in Nubaria, Beheira Governorate, Egypt) in addition two planting dates (the first planting date was 15 may, while the second date was 1 June) to study the range of genetic stability, the behavior responsible for the persistence cultivar, the interactions between these cultivars and all different environments and their effect on some yield components, respectively. Stability analysis was done using data calculated obtained from plant height, first pod height, number of pods/plant, 1000-grain weight, number of grains/plant, grain weight/plant and grain yield/plant traits beside (Random amplified polymorphic DNA (RAPD) through using four primers for the previous seven  cultivars. The final results revealed that mean square variances of (Genotypes x Environments), (Environments+(Genotypes x Environments), the linear components of (Environments) and (Genotypes x Environments) and pooled deviation were highly significant and detected that the genotypes; (Crawford, Giza 21, Giza 22, Giza 35, Giza 83, Giza 111) were the most genetic stability entries according to the results of stability analysis specially (bi, S2di, R2) and recommended for using it under different environments, while the cultivar (Giza 82) coming in the second rank. Heritability in broad sense was high in plant height, number of pods/plant and grain yield/plant traits which indicated that the environment effect on these traits through using the previous entries was very low with intension the genetic stability unlike the rest of traits studied. DNA Fingerprinting analysis was conducted to compare between the seven soybean entries using four primers namely; (OPC10, OPF-4, OPA-17, and OPG-5). The four primers recorded 12 amplicons, where 8 of them were polymorphic with 66.67% polymorphism, while 4 fragments were monomorphic. Cluster analysis divided the seven genotypes into two main clusters, where the first one contained the genotypes (2, 4), while the second cluster involved the rest of genotypes, respectively.


Keywords: Soybean,Stability analysis, Yield and its components, RAPD-PCR analysis.
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How to cite this article:

El-Mouhamady, A.A., H.M. Abdel-Rahman, T.A. Elewa and Aboud, K.A. 2017. Molecular Characterization and Stability Analysis for Yield and its Components Traits in Soybean (Glycine Max L.).Int.J.Curr.Microbiol.App.Sci. 6(7): 1835-1857. doi: https://doi.org/10.20546/ijcmas.2017.607.221