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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2256-2268
DOI: https://doi.org/10.20546/ijcmas.2017.607.326


Assessment of Antibacterial Activity of Pediocin NCDC252 Produced from Pediococus acidilactici NCDC252 and Study of Its Effect on Physico-chemical Properties of Chicken Carcasses Stored at Refrigeration Temperature
Pooja K. Kamble1, V. Appa Rao2*, Robinson J. J. Abraham2 and B. Dhanalakshmi3
1Livestock Development Officer Grade 1, Veterinary Dispensary Pratapur, Tq.Nawapur, Nandurbar – 425418, Maharashtra, India
2Department of Meat Science and Technology, Madras Veterinary College,Chennai, Tamil Nadu, India
3Department of Dairy Science, Madras Veterinary College, Chennai, Tamil Nadu, India
*Corresponding author
Abstract:

The crude pediocin NCDC252 in the form of cell free supernatant was prepared from Pediococcus acidilactici NCDC252. Culture was grown in MRS broth for 16-18 hrs and centrifuged at 12000xg for 20 minutes at 4˚C. The pH of the supernatant was adjusted to 6.5 and catalase was added (5mg/ml). Antibacterial activity of pediocin produced by Pediococcus acidilactici was assessed against Staph. Aureus and E. coli by agar well diffusion. It showed antibacterial activity against Staph aureus but not against E. coli. A study on the effect of pediocin alone and with a chelator, disodium EDTA on physico-chemical properties of chicken carcasses stored at 4 ± 1˚C was carried out and compared with control without any treatment. The quality parameters like pH, water holding capacity, extract release volume, thiobarbituric acid value, tyrosine value, Hunter color LAB analysis were analyzed and discussed, pH showed non-significant difference during storage period except on 5th day, pediocin NCDC252 with disodium EDTA treated carcasses showed lower pH than other two groups. Water holding capacity was significantly higher for treated groups compared to control group on 3rd and 5th day. On 5th and 6th day pediocin NCDC252 with disodium EDTA treated group showed significantly higher (P<0.01) ERV values compared to other two groups. Pediocin NCDC252 alone and pediocin NCDC252 with disodium EDTA treated groups showed significantly lower (P<0.01) thiobarbituric acid values than control group. On 6th day highly significant difference (P<0.01) in tyrosine value was noticed in all three groups. There was no significant difference in Hunter color LAB- L* and a* value in between treatments and during storage period. There was no significant difference in b* values in between treatments but a significant increase were noticed on 6th day in pediocin NCDC252 with disodium EDTA treated and control group.


Keywords: Pediocin, Pediococcus acidilactici, Cell Free Supernatant, Chicken Carcass, EDTA.
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How to cite this article:

Pooja K. Kamble, V. Appa Rao, Robinson J.J. Abraham and Dhanalakshmi, B. 2017. Assessment of Antibacterial Activity of Pediocin NCDC252 Produced from Pediococus acidilactici NCDC252 and Study of its Effect on Physico-chemical Properties of Chicken Carcasses Stored at Refrigeration Temperature.Int.J.Curr.Microbiol.App.Sci. 6(7): 2256-2268. doi: https://doi.org/10.20546/ijcmas.2017.607.326