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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2320-2326
DOI: https://doi.org/10.20546/ijcmas.2017.607.274


cry Gene Profile of Native Entomopathogenic Bacillus thuringiensis from Soil
A.S. Patel1*, H.N. Shelat1 and H.K. Patel2
1Department of Agricultural Microbiology, BA College of Agriculture,
2AICRP Weed Management, Anand Agricultural University, Anand (GJ), India
*Corresponding author
Abstract:

Bacillus thuringiensis produces different insecticidal crystal proteins which are encoded by cry genes. Isolation was carried out from 17 diverse locations’ soil; nine bacterial isolates were separated and selected on the basis of presence of parasporal body. Isolate ABt 10 was screened for different cry genes with universal and gene specific primers. Results indicated the presence of cry1Ac and cry1Ab, cry 4, and cry 1D genes in native isolate ABt 10. All these clearly indicated the specificity of these isolates for Lepidopteran insect pests. The search and selection of native isolates will be useful in the generation of a transgenic plant with an indigenous cry gene that offers resistance to the serious local pests.


Keywords: Bacillus thuringiensis, cry gene, H. armigera and S. litura.
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How to cite this article:

Patel, A.S., H.N. Shelat and Patel, H.K. 2017. cry Gene Profile of Native Entomopathogenic Bacillus thuringiensis from Soil.Int.J.Curr.Microbiol.App.Sci. 6(7): 2320-2326. doi: https://doi.org/10.20546/ijcmas.2017.607.274