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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 186-192
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.023


Microbial Characterization and Anti-microbial Properties of Cowhorn Silica Manure Controlling Rice Pathogens
Sivasankari Jayachandran, Unnamalai Narayanan, Ananthi Selvaraj, Arunkumar Jayaraman and Perumal Karuppan*
Shri AMM MurugappaChettiar Research Centre, Taramani, Chennai-600113, India
*Corresponding author
Abstract:

The biodynamic agriculture emphasizes the use of cowhorn silica manure (BD 501) for the improvement of growth and enhances quality and yield of crop plants. In this study, the microbial load in BD 501 manure was enumerated and evaluated for its antagonistic properties against selected rice pathogens. Three predominant bacterial isolates, BD (A)S1, BD(A)S2 and BD(A)S3 were isolated, purified, characterized and identified through microscopical, biochemical and 16S rDNA sequencing methods. These bacterial isolates were identified as Bacillus spp. based on the microscopic observation, Gram staining and biochemical tests. At species level, BD (A)S1 and BD(A)S2 isolates were identified as Bacillus amyloliquefaciens and isolate BD(A)S3 was identified as Bacillus toyonensis. These 3 isolates were tested for their antimicrobial activity against rice pathogens such as Rhizoctonia solani, Pyricularia oryzae and Xanthomonas oryzae, the causal organism of sheath blight, blast and bacterial blight of rice plants respectively. The bacterial isolate Bacillusamyloliquefaciensrecorded for a strong antifungal activity against R.solani and moderate activity against Pyricularia oryzae. This study provides a basis for confirming/ understanding the beneficial effect of BD 501.


Keywords: Cow horn silica, BD 501, Antimicrobial property, Bacillus amyloliquefaciens, Biodynamic agriculture.
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How to cite this article:

Sivasankari Jayachandran, Unnamalai Narayanan, Ananthi Selvaraj, Arunkumar Jayaraman and Perumal Karuppan. 2016. Microbial Characterization and Anti-microbial Properties of Cowhorn Silica Manure Controlling Rice Pathogens.Int.J.Curr.Microbiol.App.Sci. 5(4): 186-192. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.023