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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 1356-1365
DOI: https://doi.org/10.20546/ijcmas.2017.607.162


Fixation of Lethal Dose50 and Effect of Mutagens in M1 Generation under Laboratory Condition
K.S. Usharani1*, C.R. Ananda Kumar1 and C. Vanniarajan2
1Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University,Coimbatore - 641 003, India
2Department of Plant Breeding and Genetics, Agricultural College and Research Institute,Tamil Nadu Agricultural University, Madurai - 625 104, India
*Corresponding author
Abstract:

The current study was conducted to find out the LD50 dose, shoot length and root length induced by both gamma rays and EMS in blackgram variety VBN 4, under laboratory condition. The investigation was carried out by exposing the seeds with different doses of gamma rays (10, 20, 30, 40, 50, 60, 70, 80 and 90kR) and various concentrations of EMS (30, 40, 50, 60 and 70mM) along with the control. The effect of mutagens on germination and seedling traits like, shoot and root length was observed on 5th day after sowing. The germination percentage and seedling growth decreased with increase in doses/concentrations of the mutagens. The lethal dose 50 (LD50) was found at 50kR of gamma rays and 60mM of EMS treatments.


Keywords: Blackgram, M1 generation, Gamma rays, EMS, Germination.
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How to cite this article:

Usharani, K.S., C.R. Ananda Kumar and Vanniarajan, C. 2017. Fixation of Lethal Dose50 and Effect of Mutagens in M1 Generation under Laboratory Condition.Int.J.Curr.Microbiol.App.Sci. 6(7): 1356-1365. doi: https://doi.org/10.20546/ijcmas.2017.607.162