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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 159-164
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.020


Speciation and Detection of Virulence Factors of Coagulase Negative Staphylococci Isolated from Various Clinical Samples
K.Punitha Valli, S. Pramodhini*, S. Umadevi and K. S. Seetha
Department of Microbiology, Mahatma Gandhi Medical College and Research Institute,
Sree Balaji Vidyapeeth University, Puducherry, India
*Corresponding author
Abstract:

Coagulase negative Staphylococci (CoNS), previously dismissed as contaminants are now emerging as important potential pathogens with the increase in number of severely debilitated patients and increased use of implants in hospitals. The objectives of this study are to isolate, speciate and to detect various virulence factors of CONS from various clinical samples. A total of 500 various clinical samples from blood, urine, pus, central line tip and UVC tip were studied for a period of one year. Out of which, 62 isolates of clinically significant, non repetitive coagulase negative Staphylococci were identified by standard biochemical test. Following identification and speciation, the organism was studied for various virulence factors like Slime production, DNAse and Haemagglutination.A total of 62 CoNS, isolated from 500different clinical samples. Among various species of CoNS,S. epidermidis was most frequently isolated, corresponding to 20 (32.3%) isolates. Most of the isolates were positive for slime production (72.6%). Production of DNAse and Haemagglutination activity were shown in 43.5% and 33.9% respectively. Because of the increasing clinical significance of CoNS, accurate species identification of CoNS is highly desirable to permit a more precise determination of host-pathogen relationship of CoNS and to report them as human pathogen.


Keywords: CoNS,Slime production,DNAse,Haem-agglutination
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How to cite this article:

Punitha Valli, K., S. Pramodhini, S. Umadevi and Seetha, K. S. 2016.  Speciation and Detection of Virulence Factors of Coagulase Negative Staphylococci Isolated from Various Clinical Samples.Int.J.Curr.Microbiol.App.Sci. 5(4): 159-164. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.020