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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
coli is one of the most common pathogen that could be isolated from intestinal and from many extra intestinal infections. They can cause extra intestinal infections in both community and hospitalised patients. These extra intestinal pathogenic Escherichia coli strains exhibit certain virulence factors. The present study was undertaken to know the incidence of Escherichia coli in symptomatic cases of extra intestinal infection, to detect the virulence factors of extra intestinal pathogenic E.coli& to know the antimicrobial susceptibility pattern of extra intestinal Escherichia coli isolates. A total of 100 clinical isolates of extra intestinal E.coli and 25 commensal E.coli isolated from apparently healthy individual were screened for virulence factors like cell surface hydrophobicity, haemolysin production, Serum resistance, Mannose resistant haemagglutination (MRHA) and gelatinase production by standard methods. Chi square test was used to analyse the result. Of the 100 Extra intestinal E.coli 51% of isolates showed serum resistance. Hemolysin was produced by 47%. 23% showed cell surface hydrophobicity, 20% were MRHA positive & only 2 isolates produced protease. All the four virulence factors were present in 13% of isolates. Statistically significant difference was observed for haemolysin production & statistically highly significant difference was observed for cell surface hydrophobicity &haemagglutination activity (P <0.01 and P =0.001). Majority, 91% of E.coli isolates were sensitive to amikacin and 70 to 99% were resistant to ciprofloxacin & ampicillin. ESBL producing E.coli (21%) was isolated from urine. As number of virulence factors increased the rate of ESBL production in E,coli decreased. A comprehensive study on the virulence factors of these bacterial strains is required. The treatment of E.coli infections is increasingly becoming difficult due to development of resistance against antibiotics. Therefore it is necessary to know the antibiotic susceptibility pattern of pathogenic E.coli to select the correct antibiotic (s) for the proper treatment of the infections.