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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 1160-1173
DOI: https://doi.org/10.20546/ijcmas.2017.607.141


Phenotypic and Genotypic Characterization of Enterococci from Clinical Isolates in a Tertiary Care Hospital
Mohamadiya Rizwana*, S. Parvathi and B. Appalaraju
Department of Microbiology, PSG IMSandR, Coimbatore, Tamil Nadu, India
*Corresponding author
Abstract:

Enterococci mainly being commensals in human faeces are now considered as an important cause of nosocomial infections. Of the infections, the commonly observed are urinary tract infections, abdominal infections followed by bacteremia, endocarditis and meningitis rarely. Speciation of Enterococcus aids in effective management of the infections caused by them. The initial treatment for enterococcal infections has become challenging due to development of resistance. The resistance has been observed mainly against aminoglycosides due to the presence of bi-functional gene. VRE strains are mostly isolated from patients with recurrent bacteremia, endovascular infections leading to increase deaths in the patients. To isolate and speciate enterococcal isolates from clinical samples, study the antibiotic susceptibility pattern and the genotype associated with the aminoglycoside and vancomycin resistance. Enterococcus isolates obtained from various sections were characterized by conventional phenotypic methods. The antibiotic susceptibility was studied by disc diffusion method. The resistant strains were further confirmed by MIC using automated methods following which the genotypic analysis was done. The incidence of Enterococci was 2.5% in which around 86.4% were obtained as pure isolates. Among the species E. faecalis was found to be the maximum. The urinary isolates exhibited sensitivity of around 92.3% to nitrofurantoin and among the non - urinary isolates the maximum sensitivity was for linezolid. The molecular study showed van A to be most common gene with vancomycin resistance (71.42%), and bifunctional gene among the aminoglycoside resistance (96%). Enterococcus faecalis was the commonest species isolated. The maximum number of isolates was obtained from urinary samples. The increase incidence of resistance gene to high level gentamicin could result in failure to the synergy treatment of gentamicin in combination with penicillin group of antibiotics. The emergence of vancomycin resistant strains among the clinical isolates makes treatment options more challenging.


Keywords: Enterococci, Speciation, Antibiotic resistance, High level gentamicin, Vancomycin, Bifunctional gene
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How to cite this article:

Mohamadiya Rizwana, S. Parvathi and Appalaraju, B. 2017. Phenotypic and Genotypic Characterization of Enterococci from Clinical Isolates in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 6(7): 1160-1173. doi: https://doi.org/10.20546/ijcmas.2017.607.141