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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The papilloma viruses belong to the Papilloma viridae family and new genera were described on the basis of the interspecies phylogenetic similarity. They are DNA viruses that induce the formation of benign and malignant tumors in a vast range of hosts. The main objective of the present study was to detect PV DNA and papilloma virus-associated skin diseases in domestic and wild animals. This paper analyzed nucleotide sequence similarity of animal papilloma virus types to their closest related PV types and HPV sequences deposited in the Gen Bank. Genomic DNA was extracted from 75 blood samples and 18 warts-like lesions from several wild and domestic animals of the 15 following species: Ara ararauna, Ara chloroptera, Ara macao, Bos taurus, Bubalus bubalis, Canis familiaris, Equus caballus, Felis concolor, Macaca fascicularis, Ovis aries, Panthera leo, Panthera onca, Sus scrofa, Sus domesticus and Struthio camelus. Papilloma virus-associated skin diseases as papillomatosis, adenocarcinomas, nodular formations, melanoma and fibrous epulic lesions were diagnosed by histopathology. PV DNA bands of skin biopsy and blood samples from the animals submitted to amplification of the L1 ORF were detected. About 31 biological samples of animals of the eight species Ara chloroptera, Ara macao, Bos taurus, Canis familiaris, Felis concolor, Ovis aries, Panthera onca, Struthio camelus generated viral DNA fragments PCR amplified using degenerated primer pairs FAP59/FAP64. Nucleotide sequence similarities of animal papilloma virus types to their closest related PV types and HPV sequences deposited in Gen Bank were described suggesting that the sequences share the same ancestry of the genetic evolutionary.