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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 938-951
DOI: https://doi.org/10.20546/ijcmas.2017.607.115


Papillomavirus (PV) - Associated Skin Diseases in Domestic and Wild Animals: Animal Nucleotide Sequence Identity of PV Types to their Closest Related PV and HPV Sequences Deposited in the Gen Bank
Rachel Siqueira de Queiroz Simões* and Ortrud Monika Barth
Laboratory of Morphology and Viral Morphogenesis, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, 21040-900, Rio de Janeiro, RJ, Brazil
*Corresponding author
Abstract:

The papilloma viruses belong to the Papilloma viridae family and new genera were described on the basis of the interspecies phylogenetic similarity. They are DNA viruses that induce the formation of benign and malignant tumors in a vast range of hosts. The main objective of the present study was to detect PV DNA and papilloma virus-associated skin diseases in domestic and wild animals. This paper analyzed nucleotide sequence similarity of animal papilloma virus types to their closest related PV types and HPV sequences deposited in the Gen Bank. Genomic DNA was extracted from 75 blood samples and 18 warts-like lesions from several wild and domestic animals of the 15 following species: Ara ararauna, Ara chloroptera, Ara macao, Bos taurus, Bubalus bubalis, Canis familiaris, Equus caballus, Felis concolor, Macaca fascicularis, Ovis aries, Panthera leo, Panthera onca, Sus scrofa, Sus domesticus and Struthio camelus. Papilloma virus-associated skin diseases as papillomatosis, adenocarcinomas, nodular formations, melanoma and fibrous epulic lesions were diagnosed by histopathology. PV DNA bands of skin biopsy and blood samples from the animals submitted to amplification of the L1 ORF were detected. About 31 biological samples of animals of the eight species Ara chloroptera, Ara macao, Bos taurus, Canis familiaris, Felis concolor, Ovis aries, Panthera onca, Struthio camelus generated viral DNA fragments PCR amplified using degenerated primer pairs FAP59/FAP64. Nucleotide sequence similarities of animal papilloma virus types to their closest related PV types and HPV sequences deposited in Gen Bank were described suggesting that the sequences share the same ancestry of the genetic evolutionary.


Keywords: Animal papillomavirus, Genetic diversity, Nucleotide sequence, Papillomarividae, Polymerase chain reaction.
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How to cite this article:

Dra. Rachel Siqueira de Queiroz Simões and Dra. Ortrud Monika Barth. 2017. Papillomavirus (PV) - Associated Skin Diseases in Domestic and Wild Animals: Animal Nucleotide Sequence Identity of PV Types to their Closest Related PV and HPV Sequences Deposited in the Gen Bank.Int.J.Curr.Microbiol.App.Sci. 6(7): 938-951. doi: https://doi.org/10.20546/ijcmas.2017.607.115