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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 888-894
DOI: https://doi.org/10.20546/ijcmas.2017.607.109


The CTX-M Type ESBL Gene Production by Klebsiella Species in Urinary Tract Infection
V. Mangayarkarasi1*, K. Anitha1, D. Raja Rajeswari2 and Kalaiselvi1
1Department of Microbiology, SRM University Chennai, Tamil Nadu, India
2King Institution of Preventive Medicine, Guindy, Chennai, Tamil Nadu, India
*Corresponding author
Abstract:

Urinary Tract Infection (UTI) is one among the most common type of community and hospital acquired infection. Antibiotic resistance is a deep scientific concern both in hospital and community settings. The prevalence of ESBL range from 30% to 50% and the resistance due to CTX-M is increasing drastically from 30% to 90% around the world. Aim of the study is to detect ESBL CTX-M gene in Klebsiella isolates from patients with UTI in SRM medical college, hospital and research center a tertiary care hospital for a period of 1 year. Identification of organisms was done by routine urine analysis by semi quantitative urine culture. Antimicrobial susceptibility testing with first line and second line drugs as per CLSI guidelines 2016. Phenotypic confirmation of ESBL production in Klebsiella is done by double disk diffusion method as per CLSI guidelines 2016. PCR Detection of CTX-M Gene (HELINA KIT BASED) by gel electrophoresis. Totally we have examined 150 patients mid-stream samples of which 70 samples (46.6%) were positive for bacterial culture positive. One of the frequent pathogen associated with UTI in our hospital was Klebsiella species (25.71%), most frequent Klebsiella species isolated was Klebsiella pneumoniae 17(94.4%), ESBL production was determined by phenotypic disk diffusion method (58.82%) and we have demonstrated CTX-M gene in 70.5% by conventional PCR method.


Keywords: Klebsiella, ESBL, Double Disk Diffustion Test, CTX-M.
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How to cite this article:

Mangayarkarasi, V., K. Anitha, D. Raja Rajeswari and Kalaiselvi. 2017. The CTX-M Type ESBL Gene Production by Klebsiella Species in Urinary Tract Infection.Int.J.Curr.Microbiol.App.Sci. 6(7): 888-894. doi: https://doi.org/10.20546/ijcmas.2017.607.109