National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was conducted to investigate the bioavailability of different forms and concentration of Zinc (Zn) fed to chickens and Metallotheionein (MT) expression by Real time Polymerase Chain Reaction (RT-PCR). In the present study broiler birds were fed with corn-soya based basal diet (BD) as control (T1), BD with ZnO 80 ppm (T2) and BD with 40 and 80 ppm of Zn-methionine (T3 and T4) respectively, for a period of 42 days. At day 42, six birds from each treatment were slaughtered, liver and duodenal scrapings were collected for MT expression, whereas tibia and serum were collected for Zn estimation. When compared with control, all the treatment groups had higher levels of Zn in tibia and serum and also up regulation of MT mRNA significantly. But, birds fed with Zn- methionine exhibited MT expression significantly greater than all other treatments and suggesting that Zn-met form is more bioavailable than ZnO. Subsequent commercial trails with reduced (50 %) mineral inputs via Zn-Methionine revealed equal performance characteristics as compared to inorganic Zn feeding at 100 per cent level.
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