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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 845-849
DOI: https://doi.org/10.20546/ijcmas.2017.607.103


Real Time PCR Based Expression of Metallothionein and Evaluation of Zn Bioavailability in Chickens Fed Zinc Oxide and Zinc Methionine
A.Varun1*, N. Karthikeyan1, P. Muthusamy2, A. Raja3 and S. Wilfred Ruban4
1Department of Poultry Science, Madras Veterinary College, Chennai, Tamil Nadu, India
2Post Graduate Research Institute in Animal Sciences, TANUVAS, Kattupakkam,Tamil Nadu, India
3Department of Microbiology, VCRI, Namakkal, Tamil Nadu, India
4Department of Livestock Product Technology, Veterinary College, Bengaluru, Karnataka, Indiaa
*Corresponding author
Abstract:

An experiment was conducted to investigate the bioavailability of different forms and concentration of Zinc (Zn) fed to chickens and Metallotheionein (MT) expression by Real time Polymerase Chain Reaction (RT-PCR). In the present study broiler birds were fed with corn-soya based basal diet (BD) as control (T1), BD with ZnO 80 ppm (T2) and BD with 40 and 80 ppm of Zn-methionine (T3 and T4) respectively, for a period of 42 days. At day 42, six birds from each treatment were slaughtered, liver and duodenal scrapings were collected for MT expression, whereas tibia and serum were collected for Zn estimation. When compared with control, all the treatment groups had higher levels of Zn in tibia and serum and also up regulation of MT mRNA significantly. But, birds fed with Zn- methionine exhibited MT expression significantly greater than all other treatments and suggesting that Zn-met form is more bioavailable than ZnO. Subsequent commercial trails with reduced (50 %) mineral inputs via Zn-Methionine revealed equal performance characteristics as compared to inorganic Zn feeding at 100 per cent level.


Keywords: Zinc, Bioavailability, Metallotheionein, RT-PCR, Zn-Methionine.
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How to cite this article:

Varun, A., N. Karthikeyan, P. Muthusamy, A. Raja and Wilfred Ruban, S. 2017. Real Time PCR Based Expression of Metallothionein and Evaluation of Zn Bioavailability in Chickens Fed Zinc Oxide and Zinc MethionineInt.J.Curr.Microbiol.App.Sci. 6(7): 845-849. doi: https://doi.org/10.20546/ijcmas.2017.607.103