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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2163-2173
DOI: https://doi.org/10.20546/ijcmas.2017.606.255


Physico-Chemical Characterization of a naturally occurring Hemagglutinin in the Serum of Sand Lobster, Thenus orientalis with Affinity for N-Acetylated Aminosugars
Vasudevan Vaishnavi* and Periasamy Mullainadhan
Laboratory of Pathobiology, Department of Zoology, University of Madras, Chennai-600025, Tamil Nadu, India
*Corresponding author
Abstract:

A naturally occurring serum hemagglutinin was detected in the sand lobster, Thenus orientalis. The serum hemagglutinating activity was highest with buffalo erythrocytes. Preliminary characterization of the serum hemagglutinating activity showed that the activity was independent of calcium ions. However, there was reduced activity when exposed to divalent cationchelator, such as, EDTA and the activity was effectively restored with calcium ions. The HA activity of the lobster serum was stable between pH 7 and 9 and showed thermal stability between 20 and 40°C. Haemagglutination-inhibition assays performed with several carbohydrates revealed that the serum agglutinin was specific for N - acetylated amino sugars and that the presence of the N-acetylation was essential for agglutinin-ligand interaction. Also, the haemagglutinating activity of the serum could be specifically inhibited by lipopolysaccharides from various gram negative bacteria.


Keywords: Physico-chemical, Hemagglutinin, Thenus orientalis and Preliminary
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How to cite this article:

Vasudevan Vaishnavi and Periasamy Mullainadhan. 2017. Physico-Chemical Characterization of a Naturally Occurring Hemagglutinin in the Serum of Sand Lobster, Thenus orientalis with Affinity for N-Acetylated AminosugarsInt.J.Curr.Microbiol.App.Sci. 6(6): 2163-2173. doi: https://doi.org/10.20546/ijcmas.2017.606.255