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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 289-305
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.028


Sputum Bacteriology in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease
Dalia Saad ElFeky, Heba Mohammed Elmandory, Mohamed Galal and Mohamed AbdelHakim
Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo Universirty, Egypt
Department of Chest Diseases, Police Hospital, Agouza, Egypt
Department of Chest Diseases and Tuberculosis, Faculty of Medicine, Cairo University, Egypt
*Corresponding author
Abstract:

The present study was conducted to detect the most common bacterial pathogens associated with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and their antibiogram in Egyptian patients and to assess the quantitative culture technique of washed sputum specimens. Thirty patients diagnosed as AECOPD by pulmonary functions tests and clinical findings were subjected to physical examination, chest x-ray, routine laboratory tests, as well as direct and quantitative culture of washed sputum specimens. Potential bacterial pathogens above the cutoff count were isolated in 66.6% of studied patients. The most common isolated bacterial pathogen was Streptococcus pneumoniae (43.3%) followed by methicillin-resistant Staphylococcus aureus (MRSA) (10%), Haemophilus influenzae (6.7%) and finally Acinetobacter spp and Moraxella catarrhalis (3.3% for each). Organisms were mostly sensitive to quinolones followed by ceftriaxone. Neither leucocytosis nor CRP was a good predictor of bacterial infections. There was a good correlation between gram-stained smear results and culture results. The wash technique improved the sputum smear and culture results. Bacterial infections play an important role in AECOPD. Quinolones are good therapeutic options. The wash technique could be used to improve the diagnostic value of sputum specimens.


Keywords: COPD, Acute exacerbation, sputum specimens, Wash technique, Quantitative culture, Streptococcus pneumoniae
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How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 289-305. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.028