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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 764-769
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.088


Usefulness of Modified Centrifuged Blood Smear in Diagnosis of Malaria
Shambhavi Singh*, Vivek Agwan, Bhaskar Thakuria and Molly Madan     
Department of Microbiology Subharti Medical College Subharti University, Meerut, Uttar Pradesh 250005, India
*Corresponding author
Abstract:

Malaria is a heavy burden on tropical communities, a threat to non-endemic countries, and a danger to travellers. Keeping in mind the seriousness of the condition and the current availability of various diagnostic facilities; this study is proposed to compare the peripheral blood smear examinations (thick and thin), Quantitative Buffy Coat (QBC) examination, Rapid Antigen Detection Test (RDT), and Modified Centrifuged Blood Smear (MCBS) in clinically suspected cases of malaria. The study included 1982 blood samples of patients, clinically suspected to be of Malaria. All the samples were subjected to Thin Blood Smear, Thick Blood Smear, QBC, RDT and MCBS. Results were statistically compared considering MCBS as the standard method. Out of total 1982 samples, maximum 81(4.09%) were found positive by RDT, followed by MCBS 66(3.33%), QBC 65(3.28%), thick blood smear 63(3.18%) and thin blood smear 58(2.92%). Addition of centrifugation step in MCBS increases the sensitivity of the smear technique and at the same time preserves the morphology of the parasite, giving it more specificity. MCBS is an easy, cheap and accurate technique and could be adopted for more reliable diagnosis of malaria in resource-limited settings where expensive techniques like RDT and QBC are not feasible.


Keywords: Malaria, Modified centrifuged blood smear, Quantitative buffy coat, Rapid antigen detection.
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How to cite this article:

Shambhavi Singh, Vivek Agwan, Bhaskar Thakuria and Molly Madan. 2016. Usefulness of Modified Centrifuged Blood Smear in Diagnosis of Malaria.Int.J.Curr.Microbiol.App.Sci. 5(3): 764-769. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.088