Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2018) [Effective from January 1, 2018]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2016: 92.30
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 745-763
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.087


Screening, Identification, Characterization and Production of Bacterial Lipase from Oil Spilled Soil
Mahima Golani*, Krishnan Hajela and G. P. Pandey
School of Life Sciences, Devi Ahilya University, Indore (M.P.), India
*Corresponding author
Abstract:

A bacterial strain isolated from oil spilled soil and identified as Staphylococcus chromogenes O1A by morphological, cultural, biochemical tests and 16S rDNA gene sequence analysis with maximum lipase activity (3.1) was screened on tributyrin medium. Maximum lipase production was observed at 48 h of growth (2.30 µM/min/mg), 37°C temperature (2.30 µM/min/mg) and pH 7.0 (7.96 µM/min/mg) with agitation of 130 rpm (3.02 µM/min/mg). Yeast extract (2.07 µM/min/mg) was found to be the best nitrogen source (1%). Lactose was used as a non Lipidic carbon source (1%) for optimum production of lipase (1.69 µM/min/mg). Of the natural oils, Sunflower oil was able to induce more lipase (3.87 µM/min/mg). Among the metal ions tested Ca2+ enhanced the lipase activity (1.67 µM/min/mg) and Zn2+ showed lower activity (0.28 µM/min/mg).The enzyme was found to be stable in 0.5 % solutions of different Surfactants such as SDS, Triton X-100 & Tween80. On the basis of optimized parameters the lipase activity in newly designed production medium 3 was found to be higher (18.18 µM/min/mg).


Keywords: Lipase, Screening, Staphylococcus chromogenes O1A, Characterization, Production,Oil spilled soil.
Download this article as Download

How to cite this article:

Mahima Golani, Krishnan Hajela and Pandey, G. P. 2016. Screening, Identification, Characterization and Production of Bacterial Lipase from Oil Spilled Soil.Int.J.Curr.Microbiol.App.Sci. 5(3): 745-763. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.087